BMRB Entry 7056
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7056
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Tarendeau, Franck; Boudet, Julien; Guiligay, Delphine; Mas, Philippe; Bougault, Catherine; Boulo, S.; Baudin, F.; Ruigrok, R.; Daigle, N.; Ellenberg, J.; Cusack, Stephen; Simorre, Jean-Pierre; Hart, Darren. "Structure and nuclear import function of the C-terminal domain of influenza virus polymerase PB2 subunit." Nat. Struct. Mol. Biol. 14, 229-233 (2007).
PubMed: 17310249
Assembly members:
DPDE-trans, polymer, 83 residues, 8973 Da.
Natural source: Common Name: Influenza A virus (strain A/Victoria/3/75 H3N2) Taxonomy ID: 78586 Superkingdom: Viruses Kingdom: not available Genus/species: Orthomyxoviridae Influenza A H3N2 subtype
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
DPDE-trans: GDPDESTSGVESAVLRGFLI
LGKEDRRYGPALSINELSNL
AKGEKANVLIGQGDVVLVMK
RKRDSSILTDSQTATKRIRM
AIN
- assigned_chemical_shifts
- coupling_constants
- heteronucl_NOEs
- heteronucl_T1_relaxation
- heteronucl_T2_relaxation
| Data type | Count |
| 13C chemical shifts | 716 |
| 15N chemical shifts | 166 |
| 1H chemical shifts | 1161 |
| coupling constants | 56 |
| heteronuclear NOE values | 69 |
| T1 relaxation values | 68 |
| T1rho relaxation values | 70 |
| T2 relaxation values | 69 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | DPDE-trans | 1 |
| 2 | $DPDE | . |
Entities:
Entity 1, DPDE-trans 83 residues - 8973 Da.
The presently studied DPDE biopolymer (D2-N83) corresponds to the fragment 678-759 of C-terminal domain of influenza polymerase subunit PB2. Consequently residue numbering is G677-N759 instead of G1-N83.
| 1 | GLY | ASP | PRO | ASP | GLU | SER | THR | SER | GLY | VAL | ||||
| 2 | GLU | SER | ALA | VAL | LEU | ARG | GLY | PHE | LEU | ILE | ||||
| 3 | LEU | GLY | LYS | GLU | ASP | ARG | ARG | TYR | GLY | PRO | ||||
| 4 | ALA | LEU | SER | ILE | ASN | GLU | LEU | SER | ASN | LEU | ||||
| 5 | ALA | LYS | GLY | GLU | LYS | ALA | ASN | VAL | LEU | ILE | ||||
| 6 | GLY | GLN | GLY | ASP | VAL | VAL | LEU | VAL | MET | LYS | ||||
| 7 | ARG | LYS | ARG | ASP | SER | SER | ILE | LEU | THR | ASP | ||||
| 8 | SER | GLN | THR | ALA | THR | LYS | ARG | ILE | ARG | MET | ||||
| 9 | ALA | ILE | ASN |
Entity ., $DPDE
Samples:
sample_1: DPDE-trans, [U-13C; U-15N], 0.45 ± 0.05 mM; DPDE-cis, [U-13C; U-15N], 0.15 ± 0.05 mM; sodium chloride 200 mM; Tris pH = 7.5 50 mM; H2O 90%; D2O 10%
sample_2: DPDE-trans, [U-95% 13C; U-90% 15N], 0.45 ± 0.05 mM; DPDE-cis, [U-95% 13C; U-90% 15N], 0.15 ± 0.05 mM; sodium chloride 200 mM; Tris pH = 7.5 50 mM; D2O 99.9%
conditions_1: pH: 7.5; temperature: 283 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H15N_HSQC | not available | not available | conditions_1 |
| HNCA | not available | not available | conditions_1 |
| HN(CO)CA | not available | not available | conditions_1 |
| HN(CA)CO | not available | not available | conditions_1 |
| HNCACB | not available | not available | conditions_1 |
| CBCA(CO)NH | not available | not available | conditions_1 |
| H(CCO)NH | not available | not available | conditions_1 |
| (H)C(CO)NH | not available | not available | conditions_1 |
| H(C)CH-TOCSY | not available | not available | conditions_1 |
| (H)CCH-TOCSY | not available | not available | conditions_1 |
| 1H13C_HSQC | not available | not available | conditions_1 |
| methylselective-1H13C_HSQC | not available | not available | conditions_1 |
| 13C_NOESY_HSQC | not available | not available | conditions_1 |
| 15N_NOESY_HSQC | not available | not available | conditions_1 |
| HNHA | not available | not available | conditions_1 |
| 1H15N_HSQC_t1 | not available | not available | conditions_1 |
| 1H15N_HSQC_t1rho | not available | not available | conditions_1 |
| 1H15N_HSQC_t2 | not available | not available | conditions_1 |
| methylselective-13C_NOESY_HSQC | not available | not available | conditions_1 |
| 2D-1H1H-NOESY | not available | not available | conditions_1 |
| 15N_heteronuclear_{1H}-NOE | not available | not available | conditions_1 |
| HNCO | not available | not available | conditions_1 |
Software:
NMRPipe vVersion 2.3 - Data processing and curve fitting
NMRView vVersion 5.2.2 - data analysis and peak assignments
NMR spectrometers:
- Varian Inova600 600 MHz
- Varian Inova800 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAD02350 BAD02361 BAG49630 BAG49631 BAG49632 |
| EMBL | CAA23855 CAA33357 CAA67496 CAA67497 CAD92256 |
| GB | AAA19213 AAA19214 AAA19215 AAA43126 AAA43127 |
| PIR | P3IV34 P3IV61 S04930 |
| PRF | 1607135A |
| REF | NP_040987 YP_308849 YP_308855 |
| SP | A3DRQ0 A4GBY7 A4GCJ6 A4GCK7 A4GCL8 |
| AlphaFold | A3DRQ0 A4GBY7 A4GCJ6 A4GCK7 A4GCL8 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks