BMRB Entry 6905

Title:
Solution Structure of a Zap1 Zinc-Responsive Domain Provides Insights into Metalloregulatory Transcriptional Repression in Saccharomyces cerevisiae
Deposition date:
2005-11-18
Original release date:
2006-04-27
Authors:
Wang, Zhonghua; Feng, Linda; Matskevich, Viktor; Venkataraman, Krishna; Parasuram, Priya; Laity, John
Citation:

Citation: Wang, Zhonghua; Feng, Linda; Matskevich, Viktor; Venkataraman, Krishna; Parasuram, Priya; Laity, John. "Solution Structure of a Zap1 Zinc-responsive Domain Provides Insights into Metalloregulatory Transcriptional Repression in Saccharomyces cerevisiae"  J. Mol. Biol. 357, 1167-1183 (2006).

Assembly members:

Assembly members:
Zap1-2, polymer, 64 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Vector: p21a-zzf1-2

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts184
15N chemical shifts56
1H chemical shifts101

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
164 amino acid polypeptide1
2ZINC (II) ION, 12
3ZINC (II) ION, 22

Entities:

Entity 1, 64 amino acid polypeptide 64 residues - Formula weight is not available

1   ASPLEULYSCYSLYSTRPLYSGLUCYSPRO
2   GLUSERCYSSERSERLEUPHEASPLEUGLN
3   ARGHISLEULEULYSASPHISVALSERGLN
4   ASPPHELYSHISPROMETGLUPROLEUALA
5   CYSASNTRPGLUASPCYSASPPHELEUGLY
6   ASPASPTHRCYSSERILEVALASNHISILE
7   ASNCYSGLNHIS

Entity 2, ZINC (II) ION, 1 - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: Zap1-2, [U-C13; U-15N], 0.7 mM; ZINC (II) ION 1.5 mM; MES 20 mM; TCEP 0.5 mM; DSS 0.2 mM; NaN2 1 mM

conditions_1: pH: 6.9; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D_1H-15N_HSQCsample_1not availableconditions_1
3D_HNCACBsample_1not availableconditions_1
3D_CBCA(CO)NHsample_1not availableconditions_1
3D_HNCOsample_1not availableconditions_1
3D_H(CA)CO(CA)NHsample_1not availableconditions_1
3D_HNHAsample_1not availableconditions_1

Software:

NMRView v5.0.4 - Data analysis

NMRPipe v97.027.12.56 -

NMR spectrometers:

  • Varian Inova 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks