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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6562
MolProbity Validation Chart
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NMR-STAR v3 text file.
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Citation: Ihle, Yvonne; Ohlenschlager, Oliver; Hafner, Sabine; Duchardt, Elke; Zacharias, Martin; Seitz, Simone; Zell, Roland; Ramachandran, Ramadurai; Gorlach, Matthias. "A novel cGUUAg tetraloop structure with a conserved yYNMGg-type backbone
conformation from cloverleaf 1 of bovine enterovirus 1 RNA" Nucleic Acids Res. 33, 2003-2011 (2005).
PubMed: 15814817
Assembly members:
D-loop from cloverleaf 1 of bovine enterovirus 1 RNA, polymer, 18 residues, Formula weight is not available
Natural source: Common Name: bovine enterovirus 1 Taxonomy ID: 12064 Superkingdom: Viruses Kingdom: not available Genus/species: Enterovirus bovine enterovirus 1
Experimental source: Production method: enzymatic semisynthesis
Entity Sequences (FASTA):
D-loop from cloverleaf 1 of bovine enterovirus 1 RNA: GGCGUUCGUUAGAACGUC
Data type | Count |
13C chemical shifts | 197 |
15N chemical shifts | 93 |
31P chemical shifts | 16 |
coupling constants | 17 |
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | BEVSLDap | 1 |
Entity 1, BEVSLDap 18 residues - Formula weight is not available
1 | G | G | C | G | U | U | C | G | U | U | ||||
2 | A | G | A | A | C | G | U | C |
sample_1: D-loop from cloverleaf 1 of bovine enterovirus 1 RNA, [U-15N], 1.0 mM; H2O 90%; D2O 10%; KH2PO4/K2HPO4 10 mM; KCl 40 mM; EDTA 0.2 mM
sample_2: D-loop from cloverleaf 1 of bovine enterovirus 1 RNA, [U-13C; U-15N], 0.9 mM; H2O 90%; D2O 10%; KH2PO4/K2HPO4 10 mM; KCl 40 mM; EDTA 0.2 mM
sample_3: D-loop from cloverleaf 1 of bovine enterovirus 1 RNA, [U-13C; U-15N], 0.9 mM; D2O 99.99%; KH2PO4/K2HPO4 10 mM; KCl 40 mM; EDTA 0.2 mM
sample_cond_1: ionic strength: 50 mM; pH: 6.2; pressure: 1 atm; temperature: 298 K
sample_cond_2: ionic strength: 50 mM; pH: 6.2; pressure: 1 atm; temperature: 283 K
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
(1H,15N)-HSQC | not available | not available | not available |
(1H,15N)-CPMG-NOESY | not available | not available | not available |
(1H,1H)-H5(C5C4N)H | not available | not available | not available |
(1H,1H)-HCCNH-TOCSY | not available | not available | not available |
(1H,1H)-HCCH-TOCSY (A) | not available | not available | not available |
(1H,1H)-HCCH-COSY | not available | not available | not available |
(1H,15N)-2bond-HSQC | not available | not available | not available |
(1H,13C)-H(N)CO | not available | not available | not available |
(1H,13C)-HSQC | not available | not available | not available |
3D (1H,1H,13C)-HCCH-COSY | not available | not available | not available |
3D (1H,1H,13C)-HCCH-TOCSY | not available | not available | not available |
(1H,15N)-HCN | not available | not available | not available |
3D (1H,1H,13C)-NOESY-HSQC | not available | not available | not available |
(1H,31P)-HCP-CCH-TOCSY | not available | not available | not available |
(1H,1H)-NOESY | not available | not available | not available |
HNN-COSY | not available | not available | not available |
(1H,13C)-HCCH-E.COSY | not available | not available | not available |
VNMR v6.1, rev. c - collection, processing
XEASY v1.3.9 - data analysis
CYANA v1.0.6 - structure solution
FOUND vimplemented in CYANA-1.0.6 - structure solution
OPAL v2.6 - refinement