BMRB Entry 6474

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PDB ID: 1mzk
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6474
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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

The 15N relaxation data and backbone dynamics results of CLV1 pT868 bound KI-FHA from KAPP

Deposition date:

2005-01-30

Original release date:

2005-09-08

Authors:

Ding, Zhaofeng; Lee, Gui-in; Liang, Xiangyang; Gallazzi, Fabio; Van Doren, Steven

Citation:

Citation: Ding, Zhaofeng; Lee, Gui-In; Liang, Xiangyang; Gallazzi, Fabio; Arunima, A.; Van Doren, Steven. "PhosphoThr Peptide Binding Globally Rigidifies Much of the FHA Domain from Arabidopsis Receptor Kinase-Associated Protein Phosphatase" Biochemistry 44, 10119-10134 (2005).
PubMed: 16042389

Assembly members:

Assembly members:
Kinase Interacting FHA (KI-FHA) domain from KAPP, polymer, 139 residues, 13238 Da.
CLV1 pT868 peptide, polymer, 13 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Thale cress Taxonomy ID: 3702 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Arabidopsis thaliana

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Kinase Interacting FHA (KI-FHA) domain from KAPP: GPLGSSWLFLEVIAGPAIGL QHAVNSTSSSKLPVKLGRVS PSDLALKDSEVSGKHAQITW NSTKFKWELVDMGSLNGTLV NSHSISHPDLGSRKWGNPVE LASDDIITLGTTTKVYVRIS SQNEFQIPFKIGVASDPMA
CLV1 pT868 peptide: PEYAYTLKVDEKS

Data sets:

order_parameters
- S2_parameters_label
heteronucl_T1_relaxation
- 15N_T1_set_1
- 15N_T1_set_2
heteronucl_T2_relaxation
- 15N_T2_set_1
- 15N_T2_set_2
heteronucl_NOEs
- heteronuclear_NOE_set_1
- heteronuclear_NOE_set_2

Data type	Count
T1 relaxation values	208
T2 relaxation values	212
order parameters	101

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Kinase-interacting FHA domain of KAPP	1
2	CLV1 pT868 peptide	2

Entities:

Entity 1, Kinase-interacting FHA domain of KAPP 139 residues - 13238 Da.

1

GLY

PRO

LEU

GLY

SER

TRP

LEU

PHE

LEU

2

GLU

VAL

ILE

ALA

GLY

PRO

ALA

ILE

GLY

LEU

3

GLN

HIS

ALA

VAL

ASN

SER

THR

SER

4

LYS

LEU

PRO

VAL

LYS

LEU

GLY

ARG

VAL

SER

5

PRO

SER

ASP

LEU

ALA

LEU

LYS

ASP

SER

GLU

6

VAL

SER

GLY

LYS

HIS

ALA

GLN

ILE

THR

TRP

7

ASN

SER

THR

LYS

PHE

LYS

TRP

GLU

LEU

VAL

8

ASP

MET

GLY

SER

LEU

ASN

GLY

THR

LEU

VAL

9

ASN

SER

HIS

SER

ILE

SER

HIS

PRO

ASP

LEU

10

GLY

SER

ARG

LYS

TRP

GLY

ASN

PRO

VAL

GLU

11

LEU

ALA

SER

ASP

ILE

THR

LEU

GLY

12

THR

LYS

VAL

TYR

VAL

ARG

ILE

SER

13

SER

GLN

ASN

GLU

PHE

GLN

ILE

PRO

PHE

LYS

14

ILE

GLY

VAL

ALA

SER

ASP

PRO

MET

ALA

Entity 2, CLV1 pT868 peptide 13 residues - Formula weight is not available

1

PRO

GLU

TYR

ALA

TYR

THR

LEU

LYS

VAL

ASP

2

GLU

LYS

SER

Samples:

sample_one: Kinase Interacting FHA (KI-FHA) domain from KAPP, [U-15N], 0.53 mM; CLV1 pT868 peptide 2.1 mM

condition_one: pH: 6.3; temperature: 295 K

Experiments:

Name	Sample	Sample state	Sample conditions
15N T1 relaxation	sample_one	not available	condition_one
15N T2 relaxation	sample_one	not available	condition_one
heteronuclear 1H-15N NOE	sample_one	not available	condition_one
1H-15N HSQC	sample_one	not available	condition_one

Software:

No software information available

NMR spectrometers:

Bruker DRX 500 MHz
Varian INOVA 600 MHz

REF	NP_197429
SWISS-PROT	P46014
GenBank	AAK76527 AAM51227 AAB38148
PDB	1MZK
BMRB	5564 5841
AlphaFold	P46014