BMRB Entry 5564

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5564
MolProbity Validation Chart

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Title:
1H, 13C and 15N resonance assignment of kinase-interacting FHA domain of Arabidopsis kinase associasted protein phosphatase
Deposition date:
2002-10-17
Original release date:
2003-06-26
Authors:
Lee, Gui-in; Van Doren, Steven
Citation:

Citation: Lee, Gui-in; Li, Jia; Walker, John; Van Doren, Steven. "Letter to the Editor: 1H, 13C and 15N resonance assignments of the kinase-interacting FHA domain of Arabidopsis thaliana kinase-associated protein phosphatase "  J. Biomol. NMR 25, 253-254 (2003).

Assembly members:

Assembly members:
kinase associated protein phosphatase, polymer, 139 residues, 14786 Da.

Natural source:

Natural source:   Common Name: Thale cress   Taxonomy ID: 3702   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Arabidopsis thaliana

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
kinase associated protein phosphatase: GPLGSSWLFLEVIAGPAIGL QHAVNSTSSSKLPVKLGRVS PSDLALKDSEVSGKHAQITW NSTKFKWELVDMGSLNGTLV NSHSISHPDLGSRKWGNPVE LASDDIITLGTTTKVYVRIS SQNEFQIPFKIGVASDPMA

Data sets:
Data typeCount
1H chemical shifts861
13C chemical shifts548
15N chemical shifts116

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1KAPP kinase interaction domain1

Entities:

Entity 1, KAPP kinase interaction domain 139 residues - 14786 Da.

1   GLYPROLEUGLYSERSERTRPLEUPHELEU
2   GLUVALILEALAGLYPROALAILEGLYLEU
3   GLNHISALAVALASNSERTHRSERSERSER
4   LYSLEUPROVALLYSLEUGLYARGVALSER
5   PROSERASPLEUALALEULYSASPSERGLU
6   VALSERGLYLYSHISALAGLNILETHRTRP
7   ASNSERTHRLYSPHELYSTRPGLULEUVAL
8   ASPMETGLYSERLEUASNGLYTHRLEUVAL
9   ASNSERHISSERILESERHISPROASPLEU
10   GLYSERARGLYSTRPGLYASNPROVALGLU
11   LEUALASERASPASPILEILETHRLEUGLY
12   THRTHRTHRLYSVALTYRVALARGILESER
13   SERGLNASNGLUPHEGLNILEPROPHELYS
14   ILEGLYVALALASERASPPROMETALA

Samples:

KI-FHA_sample_1: kinase associated protein phosphatase, [U-100% 13C; U-100% 15N], 0.6 mM; soduim phosphate 20 mM; NaCl 120 mM; D2O 7%

KI-FHA_sample_2: kinase associated protein phosphatase, [U-13% 13C], 0.6 mM

Ex-con1: pH: 6.3; temperature: 295 K

Experiments:

NameSampleSample stateSample conditions
15N HSQCnot availablenot availablenot available
HNCAnot availablenot availablenot available
(HA)CA(CO)NHnot availablenot availablenot available
HN(CA)HAnot availablenot availablenot available
HA(CACO)NHnot availablenot availablenot available
HNCOnot availablenot availablenot available
HACACOnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
CCA(CO)NHnot availablenot availablenot available
HCCH-TOCSYnot availablenot availablenot available
15N NOESY-HSQCnot availablenot availablenot available
13C HSMQC-NOESYnot availablenot availablenot available
13C HSQCnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • Bruker DRX 500 MHz
  • Varian INOVA 600 MHz

Related Database Links:

BMRB 5841
PDB
GB AAB38148 AAK76527 AAM51227 AED92679 AED92680
REF NP_001154720 NP_197429
SP P46014
AlphaFold P46014

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks