BMRB Entry 5841
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5841
MolProbity Validation Chart
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NMR-STAR v3 text file.
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Citation: Lee, Gui-in; Li, Jia; Walker, John; Van Doren, Steven. "1H, (13)C and (15)N Resonance Assignments of the Kinase-interacting FHA Domain
of Arabidopsis thaliana Kinase-associated Protein Phophatase" J. Biomol. NMR 25, 253-254 (2003).
PubMed: 12652139
Assembly members:
kinase associated protein phosphatase, polymer, 139 residues, Formula weight is not available
Natural source: Common Name: Thale cress Taxonomy ID: 3702 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Arabidopsis thaliana
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX6P-1
Entity Sequences (FASTA):
kinase associated protein phosphatase: GPLGSSWLFLEVIAGPAIGL
QHAVNSTSSSKLPVKLGRVS
PSDLALKDSEVSGKHAQITW
NSTKFKWELVDMGSLNGTLV
NSHSISHPDLGSRKWGNPVE
LASDDIITLGTTTKVYVRIS
SQNEFQIPFKIGVASDPMA
- order_parameters
- H_exch_rates
- H_exch_protection_factors
- heteronucl_T1_relaxation
- heteronucl_T2_relaxation
- heteronucl_NOEs
| Data type | Count |
| T1 relaxation values | 215 |
| T2 relaxation values | 208 |
| order parameters | 104 |
| H exchange rates | 43 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Kinase-interacting FHA domain of KAPP | 1 |
Entities:
Entity 1, Kinase-interacting FHA domain of KAPP 139 residues - Formula weight is not available
| 1 | GLY | PRO | LEU | GLY | SER | SER | TRP | LEU | PHE | LEU | ||||
| 2 | GLU | VAL | ILE | ALA | GLY | PRO | ALA | ILE | GLY | LEU | ||||
| 3 | GLN | HIS | ALA | VAL | ASN | SER | THR | SER | SER | SER | ||||
| 4 | LYS | LEU | PRO | VAL | LYS | LEU | GLY | ARG | VAL | SER | ||||
| 5 | PRO | SER | ASP | LEU | ALA | LEU | LYS | ASP | SER | GLU | ||||
| 6 | VAL | SER | GLY | LYS | HIS | ALA | GLN | ILE | THR | TRP | ||||
| 7 | ASN | SER | THR | LYS | PHE | LYS | TRP | GLU | LEU | VAL | ||||
| 8 | ASP | MET | GLY | SER | LEU | ASN | GLY | THR | LEU | VAL | ||||
| 9 | ASN | SER | HIS | SER | ILE | SER | HIS | PRO | ASP | LEU | ||||
| 10 | GLY | SER | ARG | LYS | TRP | GLY | ASN | PRO | VAL | GLU | ||||
| 11 | LEU | ALA | SER | ASP | ASP | ILE | ILE | THR | LEU | GLY | ||||
| 12 | THR | THR | THR | LYS | VAL | TYR | VAL | ARG | ILE | SER | ||||
| 13 | SER | GLN | ASN | GLU | PHE | GLN | ILE | PRO | PHE | LYS | ||||
| 14 | ILE | GLY | VAL | ALA | SER | ASP | PRO | MET | ALA |
Samples:
sample_one: kinase associated protein phosphatase, [U-99% 15N], 0.6 mM; NaCl 120 mM
condition_one: pH: 6.3; temperature: 298 K; ionic strength: 0.12 M
condition_two: pH: 6.3; temperature: 293 K; ionic strength: 0.12 M
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 15N T1 relaxation | sample_one | not available | not available |
| 15N T2 relaxation | sample_one | not available | not available |
| heteronuclear 1H-15N NOE | sample_one | not available | not available |
| 1H-15N HSQC | sample_one | not available | not available |
Software:
Sybyl TRIAD v6.3 - data processing
Kaleidograph for windows v3.08 - R1, R2, Hydrogen exchange data fitting
Modelfree v4.1 - for optimizing 'Lipari-Szabo model free' parameters to heteronuclear relaxation data
NMR spectrometers:
- Bruker DRX 500 MHz
- Varian INOVA 600 MHz