BMRB Entry 6468

Title:
1H, 13C and 15N backbone resonance assignment of p38 mitogen-activated protein kinase
Deposition date:
2005-01-21
Original release date:
2006-01-06
Authors:
Vogtherr, Martin; Saxena, Krishna; Grimme, Susanne; Betz, Marco; Schieborr, Ulrich; Pescatore, Barbara; Langer, Thomas; Schwalbe, Harald
Citation:

Citation: Vogtherr, Martin; Saxena, Krishna; Grimme, Susanne; Betz, Marco; Schieborr, Ulrich; Pescatore, Barbara; Langer, Thomas; Schwalbe, Harald. "NMR backbone assignment of the mitogen-activated protein (MAP) kinase p38"  J. Biomol. NMR 32, 175-175 (2005).

Assembly members:

Assembly members:
mitogen-activated protein kinase p38 alpha, polymer, 351 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: recombinant technology

Experimental source:

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
1H chemical shifts219
13C chemical shifts684
15N chemical shifts219

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1p38 alpha1

Entities:

Entity 1, p38 alpha 351 residues - Formula weight is not available

1   GLYHISASNSERGLNGLUARGPROTHRPHE
2   TYRARGGLNGLULEUASNLYSTHRILETRP
3   GLUVALPROGLUARGTYRGLNASNLEUSER
4   PROVALGLYSERGLYALATYRGLYSERVAL
5   CYSALAALAPHEASPTHRLYSTHRGLYHIS
6   ARGVALALAVALLYSLYSLEUSERARGPRO
7   PHEGLNSERILEILEHISALALYSARGTHR
8   TYRARGGLULEUARGLEULEULYSHISMET
9   LYSHISGLUASNVALILEGLYLEULEUASP
10   VALPHETHRPROALAARGSERLEUGLUGLU
11   PHEASNASPVALTYRLEUVALTHRHISLEU
12   METGLYALAASPLEUASNASNILEVALLYS
13   CYSGLNLYSLEUTHRASPASPHISVALGLN
14   PHELEUILETYRGLNILELEUARGGLYLEU
15   LYSTYRILEHISSERALAASPILEILEHIS
16   ARGASPLEULYSPROSERASNLEUALAVAL
17   ASNGLUASPCYSGLULEULYSILELEUASP
18   PHEGLYLEUALAARGHISTHRASPASPGLU
19   METTHRGLYTYRVALALATHRARGTRPTYR
20   ARGALAPROGLUILEMETLEUASNTRPMET
21   HISTYRASNGLNTHRVALASPILETRPSER
22   VALGLYCYSILEMETALAGLULEULEUTHR
23   GLYARGTHRLEUPHEPROGLYTHRASPHIS
24   ILEASPGLNLEULYSLEUILELEUARGLEU
25   VALGLYTHRPROGLYALAGLULEULEULYS
26   LYSILESERSERGLUSERALAARGASNTYR
27   ILEGLNSERLEUALAGLNMETPROLYSMET
28   ASNPHEALAASNVALPHEILEGLYALAASN
29   PROLEUALAVALASPLEULEUGLULYSMET
30   LEUVALLEUASPSERASPLYSARGILETHR
31   ALAALAGLNALALEUALAHISALATYRPHE
32   ALAGLNTYRHISASPPROASPASPGLUPRO
33   VALALAASPPROTYRASPGLNSERPHEGLU
34   SERARGASPLEULEUILEASPGLUTRPLYS
35   SERLEUTHRTYRASPGLUVALILESERPHE
36   VAL

Samples:

triple_labeled: mitogen-activated protein kinase p38 alpha, [U-2H; U-13C; U-15N], 0.7 mM; NaCl 150 mM; Hepes*HCl 50 mM; DTT 5 mM; TSP 1 mM

selectively_labeled: mitogen-activated protein kinase p38 alpha, selectively labeled, 0.2 mM

Ex-cond_1: pH: 6.8; temperature: 298 K; ionic strength: 0.3 M

Experiments:

NameSampleSample stateSample conditions
Deuterium-decoupled TROSY variants of HNCOnot availablenot availablenot available
HN(CA)COnot availablenot availablenot available
HNCAnot availablenot availablenot available
HNCACBnot availablenot availablenot available
HN(CO)CACBnot availablenot availablenot available

Software:

XEASY - assignment

NMR spectrometers:

  • Bruker DRX 800 MHz

Related Database Links:

BMRB 17471 17940 19930 19934 19935 19936 19937
PDB
DBJ BAB85654 BAE21782 BAE30324 BAE31659 BAF84398
EMBL CAG38743
GB AAA20888 AAA57456 AAA74301 AAB51285 AAC36131
PRF 2111247A 2124426A
REF NP_001003206 NP_001136366 NP_001161985 NP_001161986 NP_036081
SP O02812 P47811 P70618 Q16539
AlphaFold Q16539

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks