BMRB Entry 17940

Title:
1H, 13C and 15N backbone resonance assignment of the activated p38 mitogen-activated protein kinase   PubMed: 22012687
Deposition date:
2011-09-15
Original release date:
2011-10-24
Authors:
Nielsen, Gerd; Schwalbe, Harald
Citation:

Citation: Nielsen, Gerd; Schwalbe, Harald. "NMR spectroscopic investigations of the activated p38 mitogen-activated protein kinase."  Chembiochem 12, 2599-2607 (2011).

Assembly members:

Assembly members:
p38alpha, polymer, 349 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET DUET

Experimental source:

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET DUET

Data sets:
Data typeCount
13C chemical shifts591
15N chemical shifts188
1H chemical shifts188

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1p38alpha1

Entities:

Entity 1, p38alpha 349 residues - Formula weight is not available

1   ASNSERGLNGLUARGPROTHRPHETYRARG
2   GLNGLULEUASNLYSTHRILETRPGLUVAL
3   PROGLUARGTYRGLNASNLEUSERPROVAL
4   GLYSERGLYALATYRGLYSERVALCYSALA
5   ALAPHEASPTHRLYSTHRGLYHISARGVAL
6   ALAVALLYSLYSLEUSERARGPROPHEGLN
7   SERILEILEHISALALYSARGTHRTYRARG
8   GLULEUARGLEULEULYSHISMETLYSHIS
9   GLUASNVALILEGLYLEULEUASPVALPHE
10   THRPROALAARGSERLEUGLUGLUPHEASN
11   ASPVALTYRLEUVALTHRHISLEUMETGLY
12   ALAASPLEUASNASNILEVALLYSCYSGLN
13   LYSLEUTHRASPASPHISVALGLNPHELEU
14   ILETYRGLNILELEUARGGLYLEULYSTYR
15   ILEHISSERALAASPILEILEHISARGASP
16   LEULYSPROSERASNLEUALAVALASNGLU
17   ASPCYSGLULEULYSILELEUASPPHEGLY
18   LEUALAARGHISTHRASPASPGLUMETTHR
19   GLYTYRVALALATHRARGTRPTYRARGALA
20   PROGLUILEMETLEUASNTRPMETHISTYR
21   ASNGLNTHRVALASPILETRPSERVALGLY
22   CYSILEMETALAGLULEULEUTHRGLYARG
23   THRLEUPHEPROGLYTHRASPHISILEASP
24   GLNLEULYSLEUILELEUARGLEUVALGLY
25   THRPROGLYALAGLULEULEULYSLYSILE
26   SERSERGLUSERALAARGASNTYRILEGLN
27   SERLEUALAGLNMETPROLYSMETASNPHE
28   ALAASNVALPHEILEGLYALAASNPROLEU
29   ALAVALASPLEULEUGLULYSMETLEUVAL
30   LEUASPSERASPLYSARGILETHRALAALA
31   GLNALALEUALAHISALATYRPHEALAGLN
32   TYRHISASPPROASPASPGLUPROVALALA
33   ASPPROTYRASPGLNSERPHEGLUSERARG
34   ASPLEULEUILEASPGLUTRPLYSSERLEU
35   THRTYRASPGLUVALILESERPHEVAL

Samples:

sample_1: p38alpha, [U-13C; U-15N; U-2H], 0.5 mM

sample_conditions_1: ionic strength: 0.15 M; pH: 6.8; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker - processing

NMR spectrometers:

  • Bruker Avance 950 MHz

Related Database Links:

BMRB 17471 19930 19934 19935 19936 19937
PDB
DBJ BAB85654 BAE21782 BAE30324 BAE31659 BAF84398
EMBL CAG38743
GB AAA20888 AAA57456 AAA74301 AAB51285 AAC36131
PRF 2111247A 2124426A
REF NP_001003206 NP_001136366 NP_001161985 NP_001161986 NP_036081
SP O02812 P47811 P70618 Q16539
AlphaFold P70618

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks