BMRB Entry 17940

Name: 1H, 13C and 15N backbone resonance assignment of the activated p38 mitogen-activated protein kinase
Published: 2011-10-24

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17940

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C and 15N backbone resonance assignment of the activated p38 mitogen-activated protein kinase

Deposition date:

2011-09-15

Original release date:

2011-10-24

Authors:

Nielsen, Gerd; Schwalbe, Harald

Citation:

Citation: Nielsen, Gerd; Schwalbe, Harald. "NMR spectroscopic investigations of the activated p38 mitogen-activated protein kinase." Chembiochem 12, 2599-2607 (2011).
PubMed: 22012687

Assembly members:

Assembly members:
p38alpha, polymer, 349 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET DUET

Entity Sequences (FASTA):

Entity Sequences (FASTA):
p38alpha: NSQERPTFYRQELNKTIWEV PERYQNLSPVGSGAYGSVCA AFDTKTGHRVAVKKLSRPFQ SIIHAKRTYRELRLLKHMKH ENVIGLLDVFTPARSLEEFN DVYLVTHLMGADLNNIVKCQ KLTDDHVQFLIYQILRGLKY IHSADIIHRDLKPSNLAVNE DCELKILDFGLARHTDDEMT GYVATRWYRAPEIMLNWMHY NQTVDIWSVGCIMAELLTGR TLFPGTDHIDQLKLILRLVG TPGAELLKKISSESARNYIQ SLAQMPKMNFANVFIGANPL AVDLLEKMLVLDSDKRITAA QALAHAYFAQYHDPDDEPVA DPYDQSFESRDLLIDEWKSL TYDEVISFV

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	591
15N chemical shifts	188
1H chemical shifts	188

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	p38alpha	1

Entities:

Entity 1, p38alpha 349 residues - Formula weight is not available

1

ASN

SER

GLN

GLU

ARG

PRO

THR

PHE

TYR

ARG

2

GLN

GLU

LEU

ASN

LYS

THR

ILE

TRP

GLU

VAL

3

PRO

GLU

ARG

TYR

GLN

ASN

LEU

SER

PRO

VAL

4

GLY

SER

GLY

ALA

TYR

GLY

SER

VAL

CYS

ALA

5

ALA

PHE

ASP

THR

LYS

THR

GLY

HIS

ARG

VAL

6

ALA

VAL

LYS

LEU

SER

ARG

PRO

PHE

GLN

7

SER

ILE

HIS

ALA

LYS

ARG

THR

TYR

ARG

8

GLU

LEU

ARG

LEU

LYS

HIS

MET

LYS

HIS

9

GLU

ASN

VAL

ILE

GLY

LEU

ASP

VAL

PHE

10

THR

PRO

ALA

ARG

SER

LEU

GLU

PHE

ASN

11

ASP

VAL

TYR

LEU

VAL

THR

HIS

LEU

MET

GLY

12

ALA

ASP

LEU

ASN

ILE

VAL

LYS

CYS

GLN

13

LYS

LEU

THR

ASP

HIS

VAL

GLN

PHE

LEU

14

ILE

TYR

GLN

ILE

LEU

ARG

GLY

LEU

LYS

TYR

15

ILE

HIS

SER

ALA

ASP

ILE

HIS

ARG

ASP

16

LEU

LYS

PRO

SER

ASN

LEU

ALA

VAL

ASN

GLU

17

ASP

CYS

GLU

LEU

LYS

ILE

LEU

ASP

PHE

GLY

18

LEU

ALA

ARG

HIS

THR

ASP

GLU

MET

THR

19

GLY

TYR

VAL

ALA

THR

ARG

TRP

TYR

ARG

ALA

20

PRO

GLU

ILE

MET

LEU

ASN

TRP

MET

HIS

TYR

21

ASN

GLN

THR

VAL

ASP

ILE

TRP

SER

VAL

GLY

22

CYS

ILE

MET

ALA

GLU

LEU

THR

GLY

ARG

23

THR

LEU

PHE

PRO

GLY

THR

ASP

HIS

ILE

ASP

24

GLN

LEU

LYS

LEU

ILE

LEU

ARG

LEU

VAL

GLY

25

THR

PRO

GLY

ALA

GLU

LEU

LYS

ILE

26

SER

GLU

SER

ALA

ARG

ASN

TYR

ILE

GLN

27

SER

LEU

ALA

GLN

MET

PRO

LYS

MET

ASN

PHE

28

ALA

ASN

VAL

PHE

ILE

GLY

ALA

ASN

PRO

LEU

29

ALA

VAL

ASP

LEU

GLU

LYS

MET

LEU

VAL

30

LEU

ASP

SER

ASP

LYS

ARG

ILE

THR

ALA

31

GLN

ALA

LEU

ALA

HIS

ALA

TYR

PHE

ALA

GLN

32

TYR

HIS

ASP

PRO

ASP

GLU

PRO

VAL

ALA

33

ASP

PRO

TYR

ASP

GLN

SER

PHE

GLU

SER

ARG

34

ASP

LEU

ILE

ASP

GLU

TRP

LYS

SER

LEU

35

THR

TYR

ASP

GLU

VAL

ILE

SER

PHE

VAL

Samples:

sample_1: p38alpha, [U-13C; U-15N; U-2H], 0.5 mM

sample_conditions_1: ionic strength: 0.15 M; pH: 6.8; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN, Bruker - processing

NMR spectrometers:

Bruker Avance 950 MHz

Related Database Links:

BMRB	17471 19930 19934 19935 19936 19937
PDB	1A9U 1BL6 1BL7 1BMK 1DI9 1IAN 1KV1 1KV2 1LEW 1LEZ 1M7Q 1OUK 1OUY 1OVE 1OZ1 1P38 1R39 1R3C 1W7H 1W82 1W83 1W84 1WBN 1WBO 1WBS 1WBT 1WBV 1WBW 1WFC 1YQJ 1YW2 1YWR 1ZYJ 1ZZ2 1ZZL 2BAJ 2BAK 2BAL 2BAQ 2EWA 2FSL 2FSM 2FSO 2FST 2GFS 2GHL 2GHM 2GTM 2GTN 2I0H 2LGC 2NPQ 2OKR 2ONL 2OZA 2PUU 2QD9 2RG5 2RG6 2Y8O 2YIS 2YIW 2YIX 2ZAZ 2ZB0 2ZB1 3BV2 3BV3 3BX5 3C5U 3CTQ 3D7Z 3D83 3DS6 3DT1 3E92 3E93 3FC1 3FI4 3FKL 3FKN 3FKO 3FL4 3FLN 3FLQ 3FLS 3FLW 3FLY 3FLZ 3FMH 3FMJ 3FMK 3FML 3FMM 3FMN 3FSF 3FSK 3GC7 3GCP 3GCQ 3GCS 3GCU 3GCV 3GFE 3GI3 3HA8 3HEC 3HEG 3HL7 3HLL 3HP2 3HP5 3HRB 3HUB 3HUC 3HV3 3HV4 3HV5 3HV6 3HV7 3HVC 3IPH 3ITZ 3IW5 3IW6 3IW7 3IW8 3K3I 3K3J 3KF7 3KQ7 3L8S 3L8X 3LFA 3LFB 3LFC 3LFD 3LFE 3LFF 3LHJ 3MGY 3MH0 3MH1 3MH2 3MH3 3MPA 3MPT 3MVL 3MVM 3MW1 3NEW 3NNU 3NNV 3NNW 3NNX 3NWW 3O8P 3O8T 3O8U 3OBG 3OBJ 3OC1 3OCG 3OD6 3ODY 3ODZ 3OEF 3P4K 3P5K 3P78 3P79 3P7A 3P7B 3P7C 3PG3 3PY3 3QUD 3QUE 3RIN 3ROC 3S3I 3S4Q 3TG1 3U8W 3UVP 3UVQ 3UVR 3ZS5 3ZSG 3ZSH 3ZSI 3ZYA 4A9Y 4AA0 4AA4 4AA5 4AAC 4DLI 4DLJ 4E5A 4E5B 4E6A 4E6C 4E8A 4EH2 4EH3 4EH4 4EH5 4EH6 4EH7 4EH8 4EH9 4EHV 4EWQ 4F9W 4F9Y 4FA2 4GEO 4KA3 4KIN 4KIP 4KIQ 4L8M 4LOO 4LOP 4LOQ 4R3C 4TYH
DBJ	BAB85654 BAE21782 BAE30324 BAE31659 BAF84398
EMBL	CAG38743
GB	AAA20888 AAA57456 AAA74301 AAB51285 AAC36131
PRF	2111247A 2124426A
REF	NP_001003206 NP_001136366 NP_001161985 NP_001161986 NP_036081
SP	O02812 P47811 P70618 Q16539
AlphaFold	O02812 P47811 P70618 Q16539

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks