BMRB Entry 6332
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6332
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Melino, Sonia; Pennestri, Matteo; Santoprete, Alessia; Bielli, Pamela; Paci, Maurizio; Ragnini-Wilson, Antonella; Cicero, Daniel. "Letter to the Editor: Assignment of the 1H, 13C and 15N resonances
of Mlc1p from Saccharomyces cerevisiae" J. Biomol. NMR 31, 367-368 (2005).
Assembly members:
Myosin Light Chain, polymer, 149 residues, Formula weight is not available
Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccaromyces cerevisiae
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Myosin Light Chain: MSATRANKDIFTLFDKKGQG
AIAKDSLGDYLRAIGYNPTN
QLVQDIINADSSLRDASSLT
LDQITGLIEVNEKELDATTK
AKTEDFVKAFQVFDKESTGK
VSVGDLRYMLTGLGEKLTDA
EVDELLKGVEVDSNGEIDYK
KFIEDVLRQ
- assigned_chemical_shifts
- heteronucl_T1_relaxation
- heteronucl_T2_relaxation
- heteronucl_NOEs
| Data type | Count |
| 1H chemical shifts | 1010 |
| 13C chemical shifts | 664 |
| 15N chemical shifts | 155 |
| T1 relaxation values | 125 |
| T2 relaxation values | 125 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | mlc monomer | 1 |
Entities:
Entity 1, mlc monomer 149 residues - Formula weight is not available
| 1 | MET | SER | ALA | THR | ARG | ALA | ASN | LYS | ASP | ILE | ||||
| 2 | PHE | THR | LEU | PHE | ASP | LYS | LYS | GLY | GLN | GLY | ||||
| 3 | ALA | ILE | ALA | LYS | ASP | SER | LEU | GLY | ASP | TYR | ||||
| 4 | LEU | ARG | ALA | ILE | GLY | TYR | ASN | PRO | THR | ASN | ||||
| 5 | GLN | LEU | VAL | GLN | ASP | ILE | ILE | ASN | ALA | ASP | ||||
| 6 | SER | SER | LEU | ARG | ASP | ALA | SER | SER | LEU | THR | ||||
| 7 | LEU | ASP | GLN | ILE | THR | GLY | LEU | ILE | GLU | VAL | ||||
| 8 | ASN | GLU | LYS | GLU | LEU | ASP | ALA | THR | THR | LYS | ||||
| 9 | ALA | LYS | THR | GLU | ASP | PHE | VAL | LYS | ALA | PHE | ||||
| 10 | GLN | VAL | PHE | ASP | LYS | GLU | SER | THR | GLY | LYS | ||||
| 11 | VAL | SER | VAL | GLY | ASP | LEU | ARG | TYR | MET | LEU | ||||
| 12 | THR | GLY | LEU | GLY | GLU | LYS | LEU | THR | ASP | ALA | ||||
| 13 | GLU | VAL | ASP | GLU | LEU | LEU | LYS | GLY | VAL | GLU | ||||
| 14 | VAL | ASP | SER | ASN | GLY | GLU | ILE | ASP | TYR | LYS | ||||
| 15 | LYS | PHE | ILE | GLU | ASP | VAL | LEU | ARG | GLN |
Samples:
sample_mlc: Myosin Light Chain, [U-99% 13C; U-99% 15N], 0.7 mM; phosphate buffer 66 mM; NaCl 0.33 mM
Ex-cond: pH: 6.9; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N HSQC | not available | not available | not available |
| 1H-15N TOCSY | not available | not available | not available |
| 1H-15N NOESY | not available | not available | not available |
| CBCACONH | not available | not available | not available |
| CBCANH | not available | not available | not available |
| HBHACONH | not available | not available | not available |
| HNCA | not available | not available | not available |
| HNCOCA | not available | not available | not available |
| HNCO | not available | not available | not available |
| hacaCONH | not available | not available | not available |
Software:
No software information available
NMR spectrometers:
- Bruker Avance 700 MHz
Related Database Links:
| PDB | |
| DBJ | GAA23287 |
| EMBL | CAA66246 CAA96813 CAY79658 |
| GB | AHY79275 EDN62013 EDV10328 EDZ72226 EEU08150 |
| REF | NP_011409 |
| SP | P53141 |
| TPG | DAA08001 |
| AlphaFold | P53141 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks