BMRB Entry 6197

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6197
MolProbity Validation Chart

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Title:
1H, 15N and 13C assigned chemical shifts for the Myosin type I SH3 domain (Myo3) from saccharomyces cerevisiae
Deposition date:
2004-04-29
Original release date:
2004-11-08
Authors:
Musi, Valeria; Birdsall, Berry; Pastore, Annalisa
Citation:

Citation: Musi, Valeria; Birdsall, Berry; Pastore, Annalisa. "1H, 15N and 13C assigned chemical shifts for the Myosin type I SH3 domain (Myo3) from saccharomyces cerevisiae"  The BMRB entry is the only known published source for the data..

Assembly members:

Assembly members:
Src Homology-3 domain, polymer, 70 residues, 7636.5 Da.

Natural source:

Natural source:   Common Name: Baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pDEST17

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Src Homology-3 domain: GKDPKFEAAYDFPGSGSSSE LPLKKGDIVFISRDEPSGWS LAKLLDGSKEGWVPTAYMTP YKDTRNTVPV

Data sets:
Data typeCount
1H chemical shifts459
13C chemical shifts286
15N chemical shifts59

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1SH3 domain 11

Entities:

Entity 1, SH3 domain 1 70 residues - 7636.5 Da.

1   GLYLYSASPPROLYSPHEGLUALAALATYR
2   ASPPHEPROGLYSERGLYSERSERSERGLU
3   LEUPROLEULYSLYSGLYASPILEVALPHE
4   ILESERARGASPGLUPROSERGLYTRPSER
5   LEUALALYSLEULEUASPGLYSERLYSGLU
6   GLYTRPVALPROTHRALATYRMETTHRPRO
7   TYRLYSASPTHRARGASNTHRVALPROVAL

Samples:

sample_1: Src Homology-3 domain, [U-95% 13C; U-90% 15N], 0.6 mM

sample_2: Src Homology-3 domain, [U-90% 15N], 0.6 mM

sample_3: Src Homology-3 domain 0.6 mM

Ex-cond_1: pH: 6.9; temperature: 298 K; ionic strength: 0.1 M

Experiments:

NameSampleSample stateSample conditions
1H-15N NOESYnot availablenot availableEx-cond_1
1H-15N TOCSYnot availablenot availableEx-cond_1
HNHAnot availablenot availableEx-cond_1
HNHBnot availablenot availableEx-cond_1
CBCA(CO)NHnot availablenot availableEx-cond_1
CBCANHnot availablenot availableEx-cond_1
HNCOnot availablenot availableEx-cond_1
HCCH TOCSYnot availablenot availableEx-cond_1
2D NOESYnot availablenot availableEx-cond_1
2D COSYnot availablenot availableEx-cond_1
2D TOCSYnot availablenot availableEx-cond_1

Software:

NMRpipe - processing data

Sparky - assignment

Xeasy - assignment

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Varian UNITY 500 MHz
  • Varian UNITY 600 MHz
  • Varian UNITY 800 MHz

Related Database Links:

PDB
DBJ GAA24601
EMBL CAA81970 CAY80962
GB AAB34124 AHY76119 EDN60039 EDV12973 EDZ71016
REF NP_012793
SP A6ZZJ1 P36006
TPG DAA09032
AlphaFold A6ZZJ1 P36006

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks