BMRB Entry 5912

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5912
MolProbity Validation Chart

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Title:
The 3D solution structure of the C-terminal region of Ku86
Deposition date:
2003-08-20
Original release date:
2004-05-15
Authors:
Harris, R.; Esposito, D.; Sankar, A.; Maman, J.; Hinks, J.; Pearl, L.; Driscoll, P.
Citation:

Citation: Harris, R.; Esposito, D.; Sankar, A.; Maman, J.; Hinks, J.; Pearl, L.; Driscoll, P.. "The 3D solution structure of the C-terminal region of Ku86 (Ku86CTR)"  J. Mol. Biol. 335, 573-582 (2004).
PubMed: 14672664

Assembly members:

Assembly members:
C-terminal region of Ku86, polymer, 120 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: PRSET-B

Entity Sequences (FASTA):

Entity Sequences (FASTA):
C-terminal region of Ku86: GPVNPAENFRVLVKQKKASF EEASNQLINHIEQFLDTNET PYFMKSIDCIRAFREEAIKF SEEQRFNNFLKALQEKVEIK QLNHFWEIVVQDGITLITKE EASGSSVTAEEAKKFLAPKD

Data sets:
Data typeCount
1H chemical shifts847
13C chemical shifts535
15N chemical shifts121

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1SH2-SH3 adapter protein drk1

Entities:

Entity 1, SH2-SH3 adapter protein drk 120 residues - Formula weight is not available

1   GLYPROVALASNPROALAGLUASNPHEARG
2   VALLEUVALLYSGLNLYSLYSALASERPHE
3   GLUGLUALASERASNGLNLEUILEASNHIS
4   ILEGLUGLNPHELEUASPTHRASNGLUTHR
5   PROTYRPHEMETLYSSERILEASPCYSILE
6   ARGALAPHEARGGLUGLUALAILELYSPHE
7   SERGLUGLUGLNARGPHEASNASNPHELEU
8   LYSALALEUGLNGLULYSVALGLUILELYS
9   GLNLEUASNHISPHETRPGLUILEVALVAL
10   GLNASPGLYILETHRLEUILETHRLYSGLU
11   GLUALASERGLYSERSERVALTHRALAGLU
12   GLUALALYSLYSPHELEUALAPROLYSASP

Samples:

sample_1: C-terminal region of Ku86, [U-15N], 1 mM; Phosphate 20 mM; NaCl 100 mM; NaN3 1 mM; H2O 90%; D2O 10%

sample_2: C-terminal region of Ku86, [U-13C; U-15N], 1 mM; Phosphate 20 mM; NaCl 100 mM; NaN3 1 mM; H2O 90%; D2O 10%

sample_3: C-terminal region of Ku86, [U-15N], 1 mM; Phosphate 20 mM; NaCl 100 mM; NaN3 1 mM; n-octyl-penta(ethylene glycol):octanol 0.96:1 5%; H2O 90%; D2O 10%

sample_cond_1: pH: 7.0; temperature: 298 K; ionic strength: 100 mM; pressure: 1 atm

sample_cond_2: pH: 7.0; temperature: 293 K; ionic strength: 100 mM; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYnot availablenot availablenot available
3D 13C-separated NOESYnot availablenot availablenot available
IPAPnot availablenot availablenot available

Software:

ANSIG v1.0.3 - data analysis

NMRPipe v1 - processing

CNS v1.1 - refinement

VNMR v6.1B - collection

NMR spectrometers:

  • Varian INOVA 800 MHz

Related Database Links:

BMRB 5221 5907
PDB
DBJ BAD96323 BAF83429 BAH24115
EMBL CAA40736
GB AAA36154 AAA59475 AAH19027 AAH95442 AAR11279
REF NP_066964 XP_001151873 XP_003254095 XP_004033222 XP_005246893
SP P13010
AlphaFold P13010

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks