BMRB Entry 5221

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5221

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C, and 15N resonance assignments for the C-Terminal Region of Ku86 (Ku86CTR)

Deposition date:

2001-11-30

Original release date:

2002-08-22

Authors:

Harris, Richard; Maman, Joseph; Hinks, John; Sankar, Andrew; Pearl, Laurence; Driscoll, Paul

Citation:

Citation: Harris, Richard; Maman, Joseph; Hinks, John; Sankar, Andrew; Pearl, Laurence; Driscoll, Paul. "Letter to the Editor: Backbone 1H, 13C, and 15N resonance assignments for the C-Terminal Region of Ku86 (Ku86CTR) " J. Biomol. NMR 22, 373-374 (2002).
PubMed: 12018486

Assembly members:

Assembly members:
C-terminal region of Ku86, polymer, 184 residues, 20741 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET

Entity Sequences (FASTA):

Entity Sequences (FASTA):
C-terminal region of Ku86: MRGSHHHHHHGMASGTMKKK DQVTAQEIFQDNHEDGPTAK KLKTEQGGAHFSVSSLAEGS VTSVGSVNPAENFRVLVKQK KASFEEASNQLINHIEQFLD TNETPYFMKSIDCIRAFREE AIKFSEEQRFNNFLKALQEK VEIKQLNHFWEIVVQDGITL ITKEEASGSSVTAEEAKKFL APKD

Data sets:

assigned_chemical_shifts
- shift_set_1

Data type	Count
1H chemical shifts	509
13C chemical shifts	448
15N chemical shifts	108

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Ku86CTR	1

Entities:

Entity 1, Ku86CTR 184 residues - 20741 Da.

1

MET

ARG

GLY

SER

HIS

2

GLY

MET

ALA

SER

GLY

THR

MET

LYS

3

ASP

GLN

VAL

THR

ALA

GLN

GLU

ILE

PHE

GLN

4

ASP

ASN

HIS

GLU

ASP

GLY

PRO

THR

ALA

LYS

5

LYS

LEU

LYS

THR

GLU

GLN

GLY

ALA

HIS

6

PHE

SER

VAL

SER

LEU

ALA

GLU

GLY

SER

7

VAL

THR

SER

VAL

GLY

SER

VAL

ASN

PRO

ALA

8

GLU

ASN

PHE

ARG

VAL

LEU

VAL

LYS

GLN

LYS

9

LYS

ALA

SER

PHE

GLU

ALA

SER

ASN

GLN

10

LEU

ILE

ASN

HIS

ILE

GLU

GLN

PHE

LEU

ASP

11

THR

ASN

GLU

THR

PRO

TYR

PHE

MET

LYS

SER

12

ILE

ASP

CYS

ILE

ARG

ALA

PHE

ARG

GLU

13

ALA

ILE

LYS

PHE

SER

GLU

GLN

ARG

PHE

14

ASN

PHE

LEU

LYS

ALA

LEU

GLN

GLU

LYS

15

VAL

GLU

ILE

LYS

GLN

LEU

ASN

HIS

PHE

TRP

16

GLU

ILE

VAL

GLN

ASP

GLY

ILE

THR

LEU

17

ILE

THR

LYS

GLU

ALA

SER

GLY

SER

18

VAL

THR

ALA

GLU

ALA

LYS

PHE

LEU

19

ALA

PRO

LYS

ASP

Samples:

sample_1: C-terminal region of Ku86, [U-13C; U-15N], 1.0 – 1.5 mM

cond_1: pH: 8.0; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H-15N HSQC	not available	not available	not available
1H-15N TROSY	not available	not available	not available
1H-15N NOESY	not available	not available	not available
1H-15N TOCSY	not available	not available	not available
HNCO	not available	not available	not available
HN(CA)CO	not available	not available	not available
HNCA	not available	not available	not available
HN(CO)CA	not available	not available	not available
HNCACB	not available	not available	not available
CBCA(CO)NH	not available	not available	not available
H(CCO)NH-TOCSY	not available	not available	not available
(H)C(CO)NH-TOCSY	not available	not available	not available

Software:

nmrPIPE - data processing

ANSIG vv3.3 - spectral analysis

NMR spectrometers:

Varian UNITYplus 500 MHz
Varian UNITYplus 600 MHz

BMRB	5912
PDB	1Q2Z
DBJ	BAD96323 BAE01114 BAF83429 BAH24115
EMBL	CAA40736 CAH92321
GB	AAA36154 AAA59475 AAH19027 AAH95442 AAR11279
REF	NP_001126362 NP_001244295 NP_066964 XP_001151873 XP_003254095
SP	P13010
AlphaFold	P13010