BMRB Entry 5909
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5909
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Clark, Andrew; McCrary, Bradford; Edmondson, Stephen; Shriver, John. "Thermodynamics of Hydrophobic Core Packing in the Thermophile Proteins Sac7d and
Sso7d" J. Mol. Biol. ., .-..
Assembly members:
Sso7d, polymer, 64 residues, 7279 Da.
Natural source: Common Name: Sulfolobus solfataricus Brock et al Taxonomy ID: 2285 Superkingdom: Archaea Kingdom: not available Genus/species: Sulfolobus solfataricus
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Sso7d: MATVKFKYKGEEKQVDISKI
KKVWRVGKMISFTYDEGGGK
TGRGAVSEKDAPKELLQMLE
KQKK
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 62 |
| 13C chemical shifts | 10 |
| 15N chemical shifts | 62 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Sso7d subunit 1 | 1 |
Entities:
Entity 1, Sso7d subunit 1 64 residues - 7279 Da.
| 1 | MET | ALA | THR | VAL | LYS | PHE | LYS | TYR | LYS | GLY | ||||
| 2 | GLU | GLU | LYS | GLN | VAL | ASP | ILE | SER | LYS | ILE | ||||
| 3 | LYS | LYS | VAL | TRP | ARG | VAL | GLY | LYS | MET | ILE | ||||
| 4 | SER | PHE | THR | TYR | ASP | GLU | GLY | GLY | GLY | LYS | ||||
| 5 | THR | GLY | ARG | GLY | ALA | VAL | SER | GLU | LYS | ASP | ||||
| 6 | ALA | PRO | LYS | GLU | LEU | LEU | GLN | MET | LEU | GLU | ||||
| 7 | LYS | GLN | LYS | LYS |
Samples:
Sso7d_sample_1: Sso7d, [U-99% 13C; U-90% 15N], 0.58 mM; H2O 90%; D2O 10%; DSS 100 uM
pH5: pH: 5.15 na; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H,15N HSQC | Sso7d_sample_1 | not available | pH5 |
| HNCACB | Sso7d_sample_1 | not available | pH5 |
| CBCA(CO)NNH | Sso7d_sample_1 | not available | pH5 |
Software:
NMRpipe - data processing, transformation, referencing
NMRView v5 - sequential assignments
NMR spectrometers:
- Varian INOVA 500 MHz
- Varian INOVA 800 MHz
Related Database Links:
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks