BMRB Entry 5909

Title:
Backbone 1HN and 15N Chemical Shift Assignments for Sso7d
Deposition date:
2003-08-20
Original release date:
2007-07-11
Authors:
Clark, Andrew; McCrary, Bradford; Edmondson, Stephen; Shriver, John
Citation:

Citation: Clark, Andrew; McCrary, Bradford; Edmondson, Stephen; Shriver, John. "Thermodynamics of Hydrophobic Core Packing in the Thermophile Proteins Sac7d and Sso7d"  J. Mol. Biol. ., .-..

Assembly members:

Assembly members:
Sso7d, polymer, 64 residues, 7279 Da.

Natural source:

Natural source:   Common Name: Sulfolobus solfataricus Brock et al   Taxonomy ID: 2285   Superkingdom: Archaea   Kingdom: not available   Genus/species: Sulfolobus solfataricus

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Data sets:
Data typeCount
1H chemical shifts62
13C chemical shifts10
15N chemical shifts62

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Sso7d subunit 11

Entities:

Entity 1, Sso7d subunit 1 64 residues - 7279 Da.

1   METALATHRVALLYSPHELYSTYRLYSGLY
2   GLUGLULYSGLNVALASPILESERLYSILE
3   LYSLYSVALTRPARGVALGLYLYSMETILE
4   SERPHETHRTYRASPGLUGLYGLYGLYLYS
5   THRGLYARGGLYALAVALSERGLULYSASP
6   ALAPROLYSGLULEULEUGLNMETLEUGLU
7   LYSGLNLYSLYS

Samples:

Sso7d_sample_1: Sso7d, [U-99% 13C; U-90% 15N], 0.58 mM; H2O 90%; D2O 10%; DSS 100 uM

pH5: pH: 5.15 na; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
1H,15N HSQCSso7d_sample_1not availablepH5
HNCACBSso7d_sample_1not availablepH5
CBCA(CO)NNHSso7d_sample_1not availablepH5

Software:

NMRpipe - data processing, transformation, referencing

NMRView v5 - sequential assignments

NMR spectrometers:

  • Varian INOVA 500 MHz
  • Varian INOVA 800 MHz

Related Database Links:

BMRB 4570 5910
PDB
DBJ BAA28274
GB AAB25172 AAK42090 AAK42212 AAK42679 ACP34956
PRF 1908252A 2107210A
REF NP_343300 NP_343422 NP_343889 WP_009990119 WP_009992021
SP P39476 P61990 P61991
AlphaFold P39476 P61990 P61991 P39476 P61990 P61991 P39476 P61990 P61991 P39476 P61990 P61991 P39476 P61990 P61991 P39476 P61990 P61991 P39476 P61990 P61991 P39476 P61990 P61991

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks