BMRB Entry 4570
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4570
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
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RDF gzip file.
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Citation: Consonni, R.; Santomo, L.; Fusi, P.; Tortora, P.; Zetta, L.. "A Single-Point Mutation in the Extreme Heat- and Pressure-Resistant Sso7d From
Sulfolobus Solfataricus Leads to a Major Rearrangement of the Hydrophobic Core" Biochemistry 38, 12709-12717 (1999).
Assembly members:
SSO7D, polymer, 62 residues, 6958 Da.
Natural source: Common Name: Sulfolobus solfataricus Taxonomy ID: 2287 Superkingdom: Archaea Kingdom: not available Genus/species: Sulfolobus solfataricus
Experimental source: Production method: recombinant technology Host organism: ESCHERICHIA COLI
Entity Sequences (FASTA):
SSO7D: ATVKFKYKGEEKQVDISKIK
KVWRVGKMISATYDEGGGKT
GRGAVSEKDAPKELLQMLEK
QK
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 303 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Sso7d, F31A mutant | 1 |
Entities:
Entity 1, Sso7d, F31A mutant 62 residues - 6958 Da.
| 1 | ALA | THR | VAL | LYS | PHE | LYS | TYR | LYS | GLY | GLU | ||||
| 2 | GLU | LYS | GLN | VAL | ASP | ILE | SER | LYS | ILE | LYS | ||||
| 3 | LYS | VAL | TRP | ARG | VAL | GLY | LYS | MET | ILE | SER | ||||
| 4 | ALA | THR | TYR | ASP | GLU | GLY | GLY | GLY | LYS | THR | ||||
| 5 | GLY | ARG | GLY | ALA | VAL | SER | GLU | LYS | ASP | ALA | ||||
| 6 | PRO | LYS | GLU | LEU | LEU | GLN | MET | LEU | GLU | LYS | ||||
| 7 | GLN | LYS |
Samples:
sample_1: SSO7D 2.0 mM
sample_cond_1: pD: 4.5; temperature: 300 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| DQF-COSY | not available | not available | not available |
| GE-TOCSY | not available | not available | not available |
| GE-NOESY | not available | not available | not available |
Software:
FELIX v970, Molecular Simulations Inc. - resonance assignments
NMR spectrometers:
- BRUKER DMX 500 MHz