BMRB Entry 5910

Title:
Backbone 1HN and 15N Chemical Shift Assignments for Sso7d I30V
Deposition date:
2003-08-20
Original release date:
2005-05-03
Authors:
Clark, Andrew; McCrary, Bradford; Edmondson, Stephen; Shriver, John
Citation:

Citation: Clark, Andrew; McCrary, Bradford; Edmondson, Stephen; Shriver, John. "Thermodynamics of core hydrophobicity and packing in the hyperthermophile proteins Sac7d and Sso7d."  Biochemistry 43, 2840-2853 (2004).
PubMed: 15005619

Assembly members:

Assembly members:
Sso7d, polymer, 64 residues, 7265 Da.

Natural source:

Natural source:   Common Name: Sulfolobus acidocaldarius Brock et al   Taxonomy ID: 2285   Superkingdom: Archaea   Kingdom: not available   Genus/species: Sulfolobus acidocaldarius

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Data sets:
Data typeCount
15N chemical shifts62
1H chemical shifts62

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Sso7d monomer1

Entities:

Entity 1, Sso7d monomer 64 residues - 7265 Da.

1   METALATHRVALLYSPHELYSTYRLYSGLY
2   GLUGLULYSGLNVALASPILESERLYSILE
3   LYSLYSVALTRPARGVALGLYLYSMETVAL
4   SERPHETHRTYRASPGLUGLYGLYGLYLYS
5   THRGLYARGGLYALAVALSERGLULYSASP
6   ALAPROLYSGLULEULEUGLNMETLEUGLU
7   LYSGLNLYSLYS

Samples:

I30V_sample_1: Sso7d, [U-98% 15N], 1.28 mM; H2O 90%; D2O 10%; DSS 100 uM

pH5_0salt: pH: 5.07 na; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
1H,15N HSQCI30V_sample_1not availablepH5_0salt
3D HSQC-TOCSYI30V_sample_1not availablepH5_0salt
3D HSQC-NOESYI30V_sample_1not availablepH5_0salt

Software:

NMRPipe - data processing, referencing, transformation

NMRView v5 - sequential assignments

NMR spectrometers:

  • Varian VXR-S 500 MHz
  • Varian INOVA 800 MHz

Related Database Links:

BMRB 5909
PDB
DBJ BAA28274
GB AAB25172 AAK42090 AAK42212 ACP37540 ACP45250
PRF 1908252A
REF NP_343300 NP_343422 WP_009992021 YP_002828838 YP_002837172
SP P61990 P61991
AlphaFold P61990 P61991 P61990 P61991 P61990 P61991 P61990 P61991 P61990 P61991 P61990 P61991 P61990 P61991 P61990 P61991

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks