BMRB Entry 5783

Title:
1H chemical shift assignments for a mutant of human beta2-microglobulin where ARG 3 is replaced by Ala
Deposition date:
2003-04-24
Original release date:
2004-02-12
Authors:
Corazza, Alessandra; Pettirossi, Fabio; Verdone, Giuliana; Viglino, Paolo; Esposito, Gennaro
Citation:

Citation: Corazza, Alessandra; Pettirossi, Fabio; Viglino, Paolo; Verdone, Giuliana; Garcia, Julian; Dumy, Pascal; Giorgetti, Sofia; Mangione, Palma; Andreola, Alessia; Stoppini, Monica; Bellotti, Vittorio; Esposito, Gennaro. "Properties of some variants of human beta2-microglobulin and amyloidogenesis"  J. Biol. Chem. ., .-..
PubMed: 14660575

Assembly members:

Assembly members:
beta2-microglobulin, polymer, 100 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Data sets:
Data typeCount
1H chemical shifts587

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1R3Abeta2-microglobulin1

Entities:

Entity 1, R3Abeta2-microglobulin 100 residues - Formula weight is not available

1   METILEGLNALATHRPROLYSILEGLNVAL
2   TYRSERARGHISPROALAGLUASNGLYLYS
3   SERASNPHELEUASNCYSTYRVALSERGLY
4   PHEHISPROSERASPILEGLUVALASPLEU
5   LEULYSASNGLYGLUARGILEGLULYSVAL
6   GLUHISSERASPLEUSERPHESERLYSASP
7   TRPSERPHETYRLEULEUTYRTYRTHRGLU
8   PHETHRPROTHRGLULYSASPGLUTYRALA
9   CYSARGVALASNHISVALTHRLEUSERGLN
10   PROLYSILEVALLYSTRPASPARGASPMET

Samples:

sample_1: beta2-microglobulin 0.7 mM; sodium phosphate 70 mM; sodium chloride 100 mM; H2O 100%

Ex-cond_1: pH: 6.5 na; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
1H-1H TOCSYnot availablenot availablenot available
1H-1H NOESYnot availablenot availablenot available

Software:

FELIX v2000 -

NMR spectrometers:

  • Bruker Avance 500 MHz

Related Database Links:

BMRB 15480 3078 3079 5169 5782 5784
PDB
DBJ BAA35182 BAG38125
EMBL CAA23830 CAH92078
GenBank AAA87972 AAA88008 AAB25312 AAB35347 AAD48083
REF NP_001009066 NP_004039
SWISS-PROT P16213 P61769 P61770 P61771