BMRB Entry 5784

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5784

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H assignment of a human variant of beta2-microglobulin where His 31 was replaced by a Tyr

Deposition date:

2003-04-24

Original release date:

2004-02-12

Authors:

Pettirossi, Fabio; Corazza, Alessandra; Viglino, Paolo; Verdone, Giuliana; Esposito, Gennaro

Citation:

Citation: Corazza, Alessandra; Pettirossi, Fabio; Viglino, Paolo; Verdone, Giuliana; Garcia, Julian; Dumy, Pascal; Giorgetti, Sofia; Mangione, Palma; Andreola, Alessia; Stoppini, Monica; Bellotti, Vittorio; Esposito, Gennaro. "Properties of some variants of human beta2-microglobulin and amyloidogenesis" J. Biol. Chem. ., .-..
PubMed: 14660575

Assembly members:

Assembly members:
beta2-microglobulin, polymer, 100 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
beta2-microglobulin: MIQRTPKIQVYSRHPAENGK SNFLNCYVSGFYPSDIEVDL LKNGERIEKVEHSDLSFSKD WSFYLLYYTEFTPTEKDEYA CRVNHVTLSQPKIVKWDRDM

Data sets:

assigned_chemical_shifts
- shift_set_1

Data type	Count
1H chemical shifts	541

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	H31Ybeta2-microglobulin	1

Entities:

Entity 1, H31Ybeta2-microglobulin 100 residues - Formula weight is not available

1	MET	ILE	GLN	ARG	THR	PRO	LYS	ILE	GLN	VAL
2	TYR	SER	ARG	HIS	PRO	ALA	GLU	ASN	GLY	LYS
3	SER	ASN	PHE	LEU	ASN	CYS	TYR	VAL	SER	GLY
4	PHE	TYR	PRO	SER	ASP	ILE	GLU	VAL	ASP	LEU
5	LEU	LYS	ASN	GLY	GLU	ARG	ILE	GLU	LYS	VAL
6	GLU	HIS	SER	ASP	LEU	SER	PHE	SER	LYS	ASP
7	TRP	SER	PHE	TYR	LEU	LEU	TYR	TYR	THR	GLU
8	PHE	THR	PRO	THR	GLU	LYS	ASP	GLU	TYR	ALA
9	CYS	ARG	VAL	ASN	HIS	VAL	THR	LEU	SER	GLN
10	PRO	LYS	ILE	VAL	LYS	TRP	ASP	ARG	ASP	MET

Samples:

sample_1: beta2-microglobulin 0.8 mM; sodium phosphate 70 mM; sodium chloride 100 mM; H2O 100%

Ex-cond_1: pH: 6.5 na; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H-1H TOCSY	not available	not available	not available
1H-1H NOESY	not available	not available	not available

Software:

FELIX v2000 -

NMR spectrometers:

Bruker Avance 500 MHz

1	MET	ILE	GLN	ARG	THR	PRO	LYS	ILE	GLN	VAL
2	TYR	SER	ARG	HIS	PRO	ALA	GLU	ASN	GLY	LYS
3	SER	ASN	PHE	LEU	ASN	CYS	TYR	VAL	SER	GLY
4	PHE	TYR	PRO	SER	ASP	ILE	GLU	VAL	ASP	LEU
5	LEU	LYS	ASN	GLY	GLU	ARG	ILE	GLU	LYS	VAL
6	GLU	HIS	SER	ASP	LEU	SER	PHE	SER	LYS	ASP
7	TRP	SER	PHE	TYR	LEU	LEU	TYR	TYR	THR	GLU
8	PHE	THR	PRO	THR	GLU	LYS	ASP	GLU	TYR	ALA
9	CYS	ARG	VAL	ASN	HIS	VAL	THR	LEU	SER	GLN
10	PRO	LYS	ILE	VAL	LYS	TRP	ASP	ARG	ASP	MET

1	MET	ILE	GLN	ARG	THR	PRO	LYS	ILE	GLN	VAL
2	TYR	SER	ARG	HIS	PRO	ALA	GLU	ASN	GLY	LYS
3	SER	ASN	PHE	LEU	ASN	CYS	TYR	VAL	SER	GLY
4	PHE	TYR	PRO	SER	ASP	ILE	GLU	VAL	ASP	LEU
5	LEU	LYS	ASN	GLY	GLU	ARG	ILE	GLU	LYS	VAL
6	GLU	HIS	SER	ASP	LEU	SER	PHE	SER	LYS	ASP
7	TRP	SER	PHE	TYR	LEU	LEU	TYR	TYR	THR	GLU
8	PHE	THR	PRO	THR	GLU	LYS	ASP	GLU	TYR	ALA
9	CYS	ARG	VAL	ASN	HIS	VAL	THR	LEU	SER	GLN
10	PRO	LYS	ILE	VAL	LYS	TRP	ASP	ARG	ASP	MET

1	MET	ILE	GLN	ARG	THR	PRO	LYS	ILE	GLN	VAL
2	TYR	SER	ARG	HIS	PRO	ALA	GLU	ASN	GLY	LYS
3	SER	ASN	PHE	LEU	ASN	CYS	TYR	VAL	SER	GLY
4	PHE	TYR	PRO	SER	ASP	ILE	GLU	VAL	ASP	LEU
5	LEU	LYS	ASN	GLY	GLU	ARG	ILE	GLU	LYS	VAL
6	GLU	HIS	SER	ASP	LEU	SER	PHE	SER	LYS	ASP
7	TRP	SER	PHE	TYR	LEU	LEU	TYR	TYR	THR	GLU
8	PHE	THR	PRO	THR	GLU	LYS	ASP	GLU	TYR	ALA
9	CYS	ARG	VAL	ASN	HIS	VAL	THR	LEU	SER	GLN
10	PRO	LYS	ILE	VAL	LYS	TRP	ASP	ARG	ASP	MET