BMRB Entry 5393
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5393
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Rehm, Till; Mavoungou, Chrystelle; Israel, Lars; Schleicher, Michael; Holak, Tad. "Letter to the Editor: Sequence-specific (1H, 15N, 13C) resonance assignment of
the N-terminal domain of the Cyclase-associated Protein (CAP) from
Dictyostelium discoideum" J. Biomol. NMR 23, 337-338 (2002).
Assembly members:
Cyclase associated Protein, polymer, 176 residues, Formula weight is not available
Natural source: Common Name: Dictyostelium discoideum Taxonomy ID: 44689 Superkingdom: Eukaryota Kingdom: not available Genus/species: Dictyostelium discoideum
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Cyclase associated Protein: SVKEFQNLVDQHITPFVALS
KKLAPEVGNQVEQLVKAIDA
EKALINTASQSKKPSQETLL
ELIKPLNNFAAEVGKIRDSN
RSSKFFNNLSAISESIGFLS
WVVVEPTPGPHVAEMRGSAE
FYTNRILKEFKGVNQDQVDW
VSNYVNFLKDLEKYIKQYHT
TGLTWNPKGGDAKSAT
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 886 |
| 13C chemical shifts | 542 |
| 15N chemical shifts | 173 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | CAP N-terminus | 1 |
Entities:
Entity 1, CAP N-terminus 176 residues - Formula weight is not available
| 1 | SER | VAL | LYS | GLU | PHE | GLN | ASN | LEU | VAL | ASP | ||||
| 2 | GLN | HIS | ILE | THR | PRO | PHE | VAL | ALA | LEU | SER | ||||
| 3 | LYS | LYS | LEU | ALA | PRO | GLU | VAL | GLY | ASN | GLN | ||||
| 4 | VAL | GLU | GLN | LEU | VAL | LYS | ALA | ILE | ASP | ALA | ||||
| 5 | GLU | LYS | ALA | LEU | ILE | ASN | THR | ALA | SER | GLN | ||||
| 6 | SER | LYS | LYS | PRO | SER | GLN | GLU | THR | LEU | LEU | ||||
| 7 | GLU | LEU | ILE | LYS | PRO | LEU | ASN | ASN | PHE | ALA | ||||
| 8 | ALA | GLU | VAL | GLY | LYS | ILE | ARG | ASP | SER | ASN | ||||
| 9 | ARG | SER | SER | LYS | PHE | PHE | ASN | ASN | LEU | SER | ||||
| 10 | ALA | ILE | SER | GLU | SER | ILE | GLY | PHE | LEU | SER | ||||
| 11 | TRP | VAL | VAL | VAL | GLU | PRO | THR | PRO | GLY | PRO | ||||
| 12 | HIS | VAL | ALA | GLU | MET | ARG | GLY | SER | ALA | GLU | ||||
| 13 | PHE | TYR | THR | ASN | ARG | ILE | LEU | LYS | GLU | PHE | ||||
| 14 | LYS | GLY | VAL | ASN | GLN | ASP | GLN | VAL | ASP | TRP | ||||
| 15 | VAL | SER | ASN | TYR | VAL | ASN | PHE | LEU | LYS | ASP | ||||
| 16 | LEU | GLU | LYS | TYR | ILE | LYS | GLN | TYR | HIS | THR | ||||
| 17 | THR | GLY | LEU | THR | TRP | ASN | PRO | LYS | GLY | GLY | ||||
| 18 | ASP | ALA | LYS | SER | ALA | THR |
Samples:
sample_1: Cyclase associated Protein, [U-15N], 0.8 – 1.2 mM
sample_2: Cyclase associated Protein, [U-13C; U-15N], 0.9 mM
Exp-cond: pH: 7.3; temperature: 300 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H NOESY | not available | not available | not available |
| 2D 1H-13C HSQC | not available | not available | not available |
| 2D 1H-15N HSQC | not available | not available | not available |
| 3D 1H-1H-15N NOESY | not available | not available | not available |
| 3D 13C-1H-1H NOESY | not available | not available | not available |
| 3D CBCA(CO)NH | not available | not available | not available |
| 3D HNCA | not available | not available | not available |
| 3D HNCO | not available | not available | not available |
Software:
XWINNMR -
Sparky -
NMR spectrometers:
- Bruker DRX 600 MHz
- Bruker DMX 750 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks