BMRB Entry 5237

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5237

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Title:
Assignment of 1H, 13C, and 15N resonances of Type II R67 Dihydrofolate Reductase
Deposition date:
2001-12-18
Original release date:
2003-12-08
Authors:
Pitcher, Wayne; DeRose, Eugene; Mueller, Geoffrey; Howell, Elizabeth; London, Robert
Citation:

Citation: Pitcher, Wayne; DeRose, Eugene; Mueller, Geoffrey; Howell, Elizabeth; London, Robert. "NMR studies of the interaction of a type II dihydrofolate reductase with pyridine nucleotides reveal unexpected phosphatase and reductase activity"  Biochemistry 42, 11150-11160 (2003).
PubMed: 14503865

Assembly members:

Assembly members:
Dihydrofolate reductase, polymer, 78 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Dihydrofolate reductase: MERSSNEVSNPVAGNFVFPS NATFGMGDRVRKKSGAAWQG QIVGWYCTNLTPEGYAVESE AHPGSVQIYPVAALERIN

Data sets:
Data typeCount
1H chemical shifts350
13C chemical shifts161
15N chemical shifts66

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1R67 DHFR subunit A1
2R67 DHFR subunit B1
3R67 DHFR subunit C1
4R67 DHFR subunit D1

Entities:

Entity 1, R67 DHFR subunit A 78 residues - Formula weight is not available

1   METGLUARGSERSERASNGLUVALSERASN
2   PROVALALAGLYASNPHEVALPHEPROSER
3   ASNALATHRPHEGLYMETGLYASPARGVAL
4   ARGLYSLYSSERGLYALAALATRPGLNGLY
5   GLNILEVALGLYTRPTYRCYSTHRASNLEU
6   THRPROGLUGLYTYRALAVALGLUSERGLU
7   ALAHISPROGLYSERVALGLNILETYRPRO
8   VALALAALALEUGLUARGILEASN

Samples:

sample_1: Dihydrofolate reductase, [U-13C; U-15N], 0.7 mM; sodium azide 6.0 mM; sodium phosphate 25 mM

conditions_1: pH: 7.0 na; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCsample_1not availableconditions_1
1H-13C CT-HSQCsample_1not availableconditions_1
HNCACBsample_1not availableconditions_1
CBCA(CO)NHsample_1not availableconditions_1
15N-edited TOCSY-HSQCsample_1not availableconditions_1
C(CO)NHsample_1not availableconditions_1
H(CCO)NHsample_1not availableconditions_1

Software:

NMRPipe - data processing

NMRView - data analysis

NMR spectrometers:

  • Varian UnityPlus 500 MHz

Related Database Links:

PDB
EMBL CAI56200 CAQ52800 CAT00055
GB AAA26083 AAA72259 AAA82255 AAN41433 AAY33960
REF WP_000442373 WP_032490450 YP_009077699 YP_009080011 YP_009080016
SP P00383
AlphaFold P00383

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks