BMRB Entry 51900

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51900

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Title:
Backbone assignment of human Polymerase Theta Motif 1
Deposition date:
2023-04-07
Original release date:
2023-10-03
Authors:
Zinn-Justin, Sophie; Miron, Simona
Citation:

Citation: Gelot, Camille; Kovacs, Marton Tibor; Miron, Simona; Mylne, Emilie; Haan, Alexis; Boeffard-Dosierre, Liza; Ghouil, Rania; Popova, Tatiana; Dingli, Florent; Loew, Damarys; Guirouilh-Barbat, Josee; Del Nery, Elaine; Zinn-Justin, Sophie; Ceccaldi, Raphael. "Polth is phosphorylated by PLK1 to repair double-strand breaks in mitosis"  Nature 621, 415-422 (2023).
PubMed: 37674080

Assembly members:

Assembly members:
entity_1, polymer, 80 residues, 8991.04 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET22b+

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: EENGLFLKKNEVSVTDSQLN SFLQGYQTQETVKPVILLIP QKRTPTGVEGENLPVPETSL NMSDSLLFDSFSDDYLVKEQ

Data sets:
Data typeCount
13C chemical shifts233
15N chemical shifts74
1H chemical shifts74

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Pol Theta Motif 11

Entities:

Entity 1, Pol Theta Motif 1 80 residues - 8991.04 Da.

1   GLUGLUASNGLYLEUPHELEULYSLYSASN
2   GLUVALSERVALTHRASPSERGLNLEUASN
3   SERPHELEUGLNGLYTYRGLNTHRGLNGLU
4   THRVALLYSPROVALILELEULEUILEPRO
5   GLNLYSARGTHRPROTHRGLYVALGLUGLY
6   GLUASNLEUPROVALPROGLUTHRSERLEU
7   ASNMETSERASPSERLEULEUPHEASPSER
8   PHESERASPASPTYRLEUVALLYSGLUGLN

Samples:

sample_1: Pol Theta Motif 1 mM; HEPES 25 mM; NaCl 50 mM

sample_conditions_1: ionic strength: 0.050 M; pH: 7; pressure: 1 atm; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCACONHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACOsample_1isotropicsample_conditions_1
3D HNCANNHsample_1isotropicsample_conditions_1

Software:

CcpNMR - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE NEO 700 MHz

Related Database Links:

UNP O75417-1

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks