BMRB Entry 5121
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5121
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Williams, Neal; Prosselkov, Pavel; Liepinsh, Edvards; Line, Inara; Sharipo, Anatoly; Littler, Dene; Curmi, Paul; Otting, Gottfried; Dixon, Nicholas. "In vivo Protein Cyclization Promoted by a Circularly Permuted Synechocystis sp.
PCC6803 DnaB Mini-intein" J. Biol. Chem. 277, 7790-7798 (2002).
PubMed: 11742000
Assembly members:
N-terminal Domain of E. coli DnaB Helicase, polymer, 114 residues, Formula weight is not available
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Eubacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
N-terminal Domain of E. coli DnaB Helicase: MKVPPHSIEAEQSVLGGLML
DNERWDDVAERVVADDFYTR
PHRHIFTEMARLQESGSPID
LITLAESLERQGQLDSVGGF
AYLAELSKNTPSAANISAYA
DIVRERAVVREMIS
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 774 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | DnaB(24-136) | 1 |
Entities:
Entity 1, DnaB(24-136) 114 residues - Formula weight is not available
| 1 | MET | LYS | VAL | PRO | PRO | HIS | SER | ILE | GLU | ALA | ||||
| 2 | GLU | GLN | SER | VAL | LEU | GLY | GLY | LEU | MET | LEU | ||||
| 3 | ASP | ASN | GLU | ARG | TRP | ASP | ASP | VAL | ALA | GLU | ||||
| 4 | ARG | VAL | VAL | ALA | ASP | ASP | PHE | TYR | THR | ARG | ||||
| 5 | PRO | HIS | ARG | HIS | ILE | PHE | THR | GLU | MET | ALA | ||||
| 6 | ARG | LEU | GLN | GLU | SER | GLY | SER | PRO | ILE | ASP | ||||
| 7 | LEU | ILE | THR | LEU | ALA | GLU | SER | LEU | GLU | ARG | ||||
| 8 | GLN | GLY | GLN | LEU | ASP | SER | VAL | GLY | GLY | PHE | ||||
| 9 | ALA | TYR | LEU | ALA | GLU | LEU | SER | LYS | ASN | THR | ||||
| 10 | PRO | SER | ALA | ALA | ASN | ILE | SER | ALA | TYR | ALA | ||||
| 11 | ASP | ILE | VAL | ARG | GLU | ARG | ALA | VAL | VAL | ARG | ||||
| 12 | GLU | MET | ILE | SER |
Samples:
sample_1: N-terminal Domain of E. coli DnaB Helicase 0.6 mM
ex-cond_1: pH*: 7.0; temperature: 304 K; ionic strength: 0.1 M
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| NOESY | not available | not available | not available |
| TOCSY | not available | not available | not available |
| COSY | not available | not available | not available |
Software:
Prosa v3.6 - spectra processing
XEASY v1.2 - peak analysis
NMR spectrometers:
- Varian INOVA 800 MHz
- Bruker DMX 600 MHz
- Bruker DMX 500 MHz
Related Database Links:
| BMRB | 4075 4297 5122 |
| PDB | |
| DBJ | BAB38457 BAE78054 BAG66812 BAG79869 BAH61192 |
| EMBL | CAP78509 CAQ34401 CAQ91563 CAR01031 CAR05689 |
| GB | AAA23689 AAA23690 AAC43146 AAC77022 AAG59250 |
| REF | NP_290685 NP_313061 NP_418476 NP_709868 NP_756878 |
| SP | P0ACB0 P0ACB1 Q8FB22 Q8X5V3 |
| AlphaFold | P0ACB0 P0ACB1 Q8FB22 Q8X5V3 |