BMRB Entry 4297

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4297

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Chemical Shifts (1H, 13C, 15N) and J-couplings (3JHNHA, 3JNHB, 3JHAHB) for the N-terminal Domain of E. coli DnaB Helicase, DnaB(24-136)

Deposition date:

1999-01-20

Original release date:

1999-02-22

Authors:

Weigelt, Johan; Brown, Susan; Miles, Caroline; Dixon, Nicholas; Otting, Gottfried

Citation:

Citation: Weigelt, Johan; Brown, Susan; Miles, Caroline; Dixon, Nicholas; Otting, Gottfried. "NMR Structure of the N-terminal Domain of E. coli DnaB Helicase: Implications for Structure Rearrangements in the Helicase Hexamer and its Biological Function" Structure Fold Des. 7, 681-690 (1999).

Assembly members:

Assembly members:
DnaB helicase, polymer, 114 residues, Formula weight is not available

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Eubacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
DnaB helicase: MKVPPHSIEAEQSVLGGLML DNERWDDVAERVVADDFYTR PHRHIFTEMARLQESGSPID LITLAESLERQGQLDSVGGF AYLAELSKNTPSAANISAYA DIVRERAVVREMIS

Data sets:

assigned_chemical_shifts
- Chemical_shifts
coupling_constants
- J_values

Data type	Count
13C chemical shifts	453
15N chemical shifts	112
1H chemical shifts	771
coupling constants	190

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	DnaB(24-136)	1

Entities:

Entity 1, DnaB(24-136) 114 residues - Formula weight is not available

1

MET

LYS

VAL

PRO

HIS

SER

ILE

GLU

ALA

2

GLU

GLN

SER

VAL

LEU

GLY

LEU

MET

LEU

3

ASP

ASN

GLU

ARG

TRP

ASP

VAL

ALA

GLU

4

ARG

VAL

ALA

ASP

PHE

TYR

THR

ARG

5

PRO

HIS

ARG

HIS

ILE

PHE

THR

GLU

MET

ALA

6

ARG

LEU

GLN

GLU

SER

GLY

SER

PRO

ILE

ASP

7

LEU

ILE

THR

LEU

ALA

GLU

SER

LEU

GLU

ARG

8

GLN

GLY

GLN

LEU

ASP

SER

VAL

GLY

PHE

9

ALA

TYR

LEU

ALA

GLU

LEU

SER

LYS

ASN

THR

10

PRO

SER

ALA

ASN

ILE

SER

ALA

TYR

ALA

11

ASP

ILE

VAL

ARG

GLU

ARG

ALA

VAL

ARG

12

GLU

MET

ILE

SER

Samples:

sample_1: DnaB helicase, {U-98% 15N}, 2.2 mM; sodium phosphate 20 mM; EDTA 1 mM; sodiumn azide 0.02 % w/v

sample_2: DnaB helicase, {U-98% 13C, U-98% 15N}, 2.2 mM; sodium phosphate 20 mM; EDTA 1 mM; sodiumn azide 0.02 % w/v

sample_3: DnaB helicase, {U-10% 13C, U-98% 15N}, 2.2 mM; sodium phosphate 20 mM; EDTA 1 mM; sodiumn azide 0.02 % w/v

Ex-cond_1: pH: 7.5 na; temperature: 305 K

Experiments:

Name	Sample	Sample state	Sample conditions

Software:

No software information available

NMR spectrometers:

unknown unknown 0 MHz

BMRB	4075
PDB	1B79 1JWE
DBJ	BAB38457 BAE78054 BAG66812 BAG79869 BAH61192
EMBL	CAP78509 CAQ34401 CAQ91563 CAR01031 CAR05689
GB	AAA23689 AAA23690 AAC43146 AAC77022 AAG59250
REF	NP_313061 NP_418476 NP_709868 WP_000918354 WP_000918355
SP	P0ACB0 P0ACB1 Q8FB22 Q8X5V3
AlphaFold	P0ACB0 P0ACB1 Q8FB22 Q8X5V3