BMRB Entry 4075

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4075

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone NMR Assignments and Secondary Structure of the N Terminal Domain of DnaB Helicase from E. coli

Deposition date:

1997-11-25

Original release date:

1998-10-12

Authors:

Weigelt, Johan; Miles, Caroline; Dixon, Nicholas; Otting, Gottfried

Citation:

Citation: Weigelt, Johan; Miles, Caroline; Dixon, Nicholas; Otting, Gottfried. "Backbone NMR Assignments and Secondary Structure of the N Terminal Domain of DnaB Helicase from E. coli" J. Biomol. NMR 11, 233-234 (1998).

Assembly members:

Assembly members:
DnaB helicase, polymer, 142 residues, Formula weight is not available

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Eubacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pCM865

Entity Sequences (FASTA):

Entity Sequences (FASTA):
DnaB helicase: AGNKPFNKQQAEPRERDPQV AGLKVPPHSIEAEQSVLGGL MLDNERWDDVAERVVADDFY TRPHRHIFTEMARLQESGSP IDLITLAESLERQGQLDSVG GFAYLAELSKNTPSAANISA YADIVRERAVVREMISVANE IA

Data sets:

assigned_chemical_shifts
- chemical_shift_assignment

Data type	Count
13C chemical shifts	253
15N chemical shifts	124
1H chemical shifts	259

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	DnaB-Nterm	1

Entities:

Entity 1, DnaB-Nterm 142 residues - Formula weight is not available

1

ALA

GLY

ASN

LYS

PRO

PHE

ASN

LYS

GLN

2

ALA

GLU

PRO

ARG

GLU

ARG

ASP

PRO

GLN

VAL

3

ALA

GLY

LEU

LYS

VAL

PRO

HIS

SER

ILE

4

GLU

ALA

GLU

GLN

SER

VAL

LEU

GLY

LEU

5

MET

LEU

ASP

ASN

GLU

ARG

TRP

ASP

VAL

6

ALA

GLU

ARG

VAL

ALA

ASP

PHE

TYR

7

THR

ARG

PRO

HIS

ARG

HIS

ILE

PHE

THR

GLU

8

MET

ALA

ARG

LEU

GLN

GLU

SER

GLY

SER

PRO

9

ILE

ASP

LEU

ILE

THR

LEU

ALA

GLU

SER

LEU

10

GLU

ARG

GLN

GLY

GLN

LEU

ASP

SER

VAL

GLY

11

GLY

PHE

ALA

TYR

LEU

ALA

GLU

LEU

SER

LYS

12

ASN

THR

PRO

SER

ALA

ASN

ILE

SER

ALA

13

TYR

ALA

ASP

ILE

VAL

ARG

GLU

ARG

ALA

VAL

14

VAL

ARG

GLU

MET

ILE

SER

VAL

ALA

ASN

GLU

15

ILE

ALA

Samples:

sample_one: DnaB helicase, [U-13C;U-15N], 1.8 mM; sodium phosphate 20 mM; pefabloc 1 mM; EDTA 1 mM; NaN3 0.02 % w/v

sample_conditions: pH: 6.5; temperature: 305 K

Experiments:

Name	Sample	Sample state	Sample conditions

Software:

No software information available

NMR spectrometers:

unknown unknown 0 MHz

BMRB	4297
PDB	1B79 1JWE
DBJ	BAB38457 BAE78054 BAG66812 BAG79869 BAH61192
EMBL	CAP78509 CAQ34401 CAQ91563 CAR01031 CAR05689
GB	AAA23689 AAA23690 AAC43146 AAC77022 AAG59250
REF	NP_313061 NP_418476 NP_709868 WP_000918354 WP_000918355
SP	P0ACB0 P0ACB1 Q8FB22 Q8X5V3
AlphaFold	P0ACB0 P0ACB1 Q8FB22 Q8X5V3