BMRB Entry 50324

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50324

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Title:
Assigned backbone chemical shifts of DEPTOR-PDZ
Deposition date:
2020-06-12
Original release date:
2021-08-20
Authors:
Heimhalt, Maren; Berndt, Alex; Wagstaff, Jane; Perisic, Olga; Maslen, Sarah; Yu, Conny Wing-Heng; Anandapadamanaban, Madhangopal; Masson, Glenn; Boland, Andreas; Johnson, Christopher; McLaughlin, Stephen; Skehel, Mark; Freund, Stefan; Williams, Roger
Citation:

Citation: Heimhalt, Maren; Berndt, Alex; Wagstaff, Jane; Anandapadamanaban, Madhangopal; Perisic, Olga; Maslen, Sarah; McLaughlin, Stephen; Yu, Conny Wing-Heng; Masson, Glenn; Boland, Andreas; Ni, Xiaodan; Yamashita, Keitaro; Murshudov, Garib; Skehel, Mark; Freund, Stefan; Williams, Roger. "Bipartite binding and partial inhibition links DEPTOR and mTOR in a mutually antagonistic embrace"  Elife 10, e68799-e68799 (2021).
PubMed: 34519269

Assembly members:

Assembly members:
entity_1, polymer, 90 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pOPTL

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GSHMAPYARKTFTIVGDAVG WGFVVRGSKPCHIQAVDPSG PAAAAGMKVCQFVVSVNGLN VLHVDYRTVNNLILTGPRTI VMEVMEELEC

Data sets:
Data typeCount
13C chemical shifts251
15N chemical shifts80
1H chemical shifts80

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1DEPTOR-PDZ1

Entities:

Entity 1, DEPTOR-PDZ 90 residues - Formula weight is not available

This construct contains a cloning scar at the N-terminus (GSHM) the construct covers residues 324 to 409 of the published DEPTOR sequence.

1   GLYSERHISMETALAPROTYRALAARGLYS
2   THRPHETHRILEVALGLYASPALAVALGLY
3   TRPGLYPHEVALVALARGGLYSERLYSPRO
4   CYSHISILEGLNALAVALASPPROSERGLY
5   PROALAALAALAALAGLYMETLYSVALCYS
6   GLNPHEVALVALSERVALASNGLYLEUASN
7   VALLEUHISVALASPTYRARGTHRVALASN
8   ASNLEUILELEUTHRGLYPROARGTHRILE
9   VALMETGLUVALMETGLUGLULEUGLUCYS

Samples:

sample_1: DEPTOR-PDZ, [U-98% 13C; U-98% 15N], 190 uM; D2O 5% v/v; HEPES 50 mM; NaCl 200 mM

sample_conditions_1: pH: 8; pressure: 1 atm; temperature: 278 K

Experiments:

NameSampleSample stateSample conditions
115N-1H BEST-TROSYsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1

Software:

TOPSPIN v3.6.0 - collection, processing

NMRFAM-SPARKY - chemical shift assignment, data analysis

MARS - chemical shift assignment

NMR spectrometers:

  • Bruker Avance II+ 700 MHz

Related Database Links:

UniprotKB QSTB45

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks