BMRB Entry 4964

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4964

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Title:
1H, 13C and 15N resonance assignment for barnase
Deposition date:
2001-02-28
Original release date:
2002-07-26
Authors:
Bocharov, Eduard; Korzhnev, Dmitry; Zhuravlyova, Anastasya; Tischenko, Eugene; Reibarkh, Mikhail; Ermolyuk, Yaroslav; Schulga, Alexey; Kirpichnikov, Mikhail; Billeter, Martin; Arseniev, Alexander
Citation:

Citation: Korzhnev, Dmitry; Bocharov, Eduard; Zhuravlyova, Anastasya; Tischenko, Eugene; Reibarkh, Mikhail; Ermolyuk, Yaroslav; Schulga, Alexey; Kirpichnikov, Mikhail; Billeter, Martin; Arseniev, Alexander. "1H, 13C and 15N resonance assignment for barnase"  Appl. Magn. Reson. 21, 195-201 (2001).

Assembly members:

Assembly members:
barnase, polymer, 110 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Bacillus amyloliquefaciens   Taxonomy ID: 1390   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Bacillus amyloliquefaciens

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
barnase: AQVINTFDGVADYLQTYHKL PDNYITKSEAQALGWVASKG NLADVAPGKSIGGDIFSNRE GKLPGKSGRTWREADINYTS GFRNSDRILYSSDWLIYKTT DHYQTFTKIR

Data sets:
Data typeCount
1H chemical shifts769
13C chemical shifts496
15N chemical shifts118

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1barnase1

Entities:

Entity 1, barnase 110 residues - Formula weight is not available

1   ALAGLNVALILEASNTHRPHEASPGLYVAL
2   ALAASPTYRLEUGLNTHRTYRHISLYSLEU
3   PROASPASNTYRILETHRLYSSERGLUALA
4   GLNALALEUGLYTRPVALALASERLYSGLY
5   ASNLEUALAASPVALALAPROGLYLYSSER
6   ILEGLYGLYASPILEPHESERASNARGGLU
7   GLYLYSLEUPROGLYLYSSERGLYARGTHR
8   TRPARGGLUALAASPILEASNTYRTHRSER
9   GLYPHEARGASNSERASPARGILELEUTYR
10   SERSERASPTRPLEUILETYRLYSTHRTHR
11   ASPHISTYRGLNTHRPHETHRLYSILEARG

Samples:

brn1: barnase, [U-13C; U-15N; U-40% 2H], 1.2 mM

brn2: barnase, [U-15N], 1.0 mM

brn3: barnase 1.0 mM

brn1_condition: pH: 6.7; temperature: 303 K; ionic strength: 0.03 M

Experiments:

NameSampleSample stateSample conditions
15N-HSQCnot availablenot availablenot available
13C-HSQCnot availablenot availablenot available
NOESYnot availablenot availablenot available
TOCSYnot availablenot availablenot available
1H-15N NOESY-HSQCnot availablenot availablenot available
1H-15N TOCSY-HSQCnot availablenot availablenot available
HNCAnot availablenot availablenot available
HNCACBnot availablenot availablenot available
HNHAnot availablenot availablenot available
HNCOnot availablenot availablenot available
HCCH-TOCSYnot availablenot availablenot available

Software:

XEASY v1.2.11 - peak assignments

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Varian UNITY 600 MHz

Related Database Links:

BMRB 16169 16170 16171 16172 2066 2067 2070 7139 975
PDB
EMBL CAA31365 CBI44445 CCF06756 CCG51396 CDG27389
GB AAA86441 AAB28825 AAB29635 AAC53661 AAP41137
PRF 1204204A 2004243A 721946A
REF WP_003151497 WP_007407419 WP_012118533 WP_013353722 WP_014472289
SP P00648 P35078
AlphaFold P00648 P35078

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks