BMRB Entry 16172

Title:
Barnase bound to d(CGAC) high pressure
Deposition date:
2009-02-11
Original release date:
2009-09-04
Authors:
Williamson, Mike; Wilton, David
Citation:

Citation: Wilton, David; Kitahara, Ryo; Akasaka, Kazuyuki; Pandya, Maya; Williamson, Mike. "Pressure-dependent structure changes in barnase on ligand binding reveal intermediate rate fluctuations"  Biophys. J. 97, 1482-1490 (2009).
PubMed: 19720037

Assembly members:

Assembly members:
barnase, polymer, 108 residues, 12132.562 Da.

Natural source:

Natural source:   Common Name: Bacillus amyloliquefaciens   Taxonomy ID: 1390   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Bacillus amyloliquefaciens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pQE-60

Data sets:
Data typeCount
1H chemical shifts356

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1barnase1

Entities:

Entity 1, barnase 108 residues - 12132.562 Da.

1   VALILEASNTHRPHEASPGLYVALALAASP
2   TYRLEUGLNTHRTYRHISLYSLEUPROASP
3   ASNTYRILETHRLYSSERGLUALAGLNALA
4   LEUGLYTRPVALALASERLYSGLYASNLEU
5   ALAASPVALALAPROGLYLYSSERILEGLY
6   GLYASPILEPHESERASNARGGLUGLYLYS
7   LEUPROGLYLYSSERGLYARGTHRTRPARG
8   GLUALAASPILEASNTYRTHRSERGLYPHE
9   ARGASNSERASPARGILELEUTYRSERSER
10   ASPTRPLEUILETYRLYSTHRTHRASPALA
11   TYRGLNTHRPHETHRLYSILEARG

Samples:

sample_1: barnase, [U-100% 13C; U-100% 15N], 1.1 mM; sodium phosphate 30 mM; d(CGAC) 4.7 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 6.7; pressure: 2000 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1

Software:

X-PLOR, Brunger - structure solution

NMR spectrometers:

  • Bruker DRX 800 MHz

Related Database Links:

BMRB 16169 16170 16171 2066 2067 2070
PDB
EMBL CAA31365 CBI44445 CCF06756 CDG27389 COC83865
GB AAA86441 AAC53661 AAP41137 AAS44581 AAS44589
PRF 1204204A 721946A
REF WP_003151497 WP_013353722 WP_015387655 WP_045512245
SP P00648
AlphaFold P00648