BMRB Entry 2067

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR2067

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Title:
Characterization of Phosphate Binding in the Active Site of Barnase by Site-Directed Mutagenesis and NMR
Deposition date:
1995-07-31
Original release date:
1995-07-31
Authors:
Meiering, Elizabeth; Bycroft, Mark; Fersht, Alan
Citation:

Citation: Meiering, Elizabeth; Bycroft, Mark; Fersht, Alan. "Characterization of Phosphate Binding in the Active Site of Barnase by Site-Directed Mutagenesis and NMR"  Biochemistry 30, 11348-11356 (1991).

Assembly members:

Assembly members:
barnase, polymer, 110 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: 1390   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Bacillus amyloliquefaciens

Experimental source:

Experimental source:   Production method: not available

Entity Sequences (FASTA):

Entity Sequences (FASTA):
barnase: AQVINTFDGVADYLQTYHKL PDNYITKSEAQALGWVASKG NLADVAPGKSIGGDIFSNRE GKLPGKSGRTWREADINYTS GFRNSDRILYSSDWLIYKTT DHYQTFTKIR

Data sets:
Data typeCount
1H chemical shifts76

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1barnase1

Entities:

Entity 1, barnase 110 residues - Formula weight is not available

1   ALAGLNVALILEASNTHRPHEASPGLYVAL
2   ALAASPTYRLEUGLNTHRTYRHISLYSLEU
3   PROASPASNTYRILETHRLYSSERGLUALA
4   GLNALALEUGLYTRPVALALASERLYSGLY
5   ASNLEUALAASPVALALAPROGLYLYSSER
6   ILEGLYGLYASPILEPHESERASNARGGLU
7   GLYLYSLEUPROGLYLYSSERGLYARGTHR
8   TRPARGGLUALAASPILEASNTYRTHRSER
9   GLYPHEARGASNSERASPARGILELEUTYR
10   SERSERASPTRPLEUILETYRLYSTHRTHR
11   ASPHISTYRGLNTHRPHETHRLYSILEARG

Related Database Links:

BMRB 16169 16170 16171 16172 2066 2070
PDB 1A2P 1B20 1B21 1B27 1B2S 1B2U 1B2X 1B2Z 1B3S 1BAN 1BAO 1BGS 1BNE 1BNF 1BNG 1BNI 1BNJ 1BNR 1BNS 1BRG 1BRH 1BRI 1BRJ 1BRK 1BRN 1BRS 1BSA 1BSB 1BSC 1BSD 1BSE 1FW7 1RNB 1X1U 1X1W 1X1X 1X1Y 1YVS 2C4B 2F4Y 2F56 2F5M 2F5W 2KF3 2KF4 2KF5 2KF6 2ZA4 3KCH
EMBL CAA31365 CBI44445 CCF06756 CCG51396 CDG27389
GB AAA86441 AAB28825 AAB29635 AAC53661 AAP41137
PRF 1204204A 2004243A 721946A
REF WP_003151497 WP_007407419 WP_013353722 WP_015387655 WP_016937826
SP P00648 P35078
AlphaFold P00648 P35078