BMRB Entry 4901

Name: 1H, 15N, 13C assignments of the N-terminal domain of the human TFIIH p62 subunit
Published: 2001-04-26

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR4901

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 15N, 13C assignments of the N-terminal domain of the human TFIIH p62 subunit

Deposition date:

2000-11-22

Original release date:

2001-04-26

Authors:

Gervais, Virginie; Lamour, Valerie; Gaudin, Florence; Thierry, Jean-Claude; Kieffer, Bruno

Citation:

Citation: Gervais, Virginie; Lamour, Valerie; Gaudin, Florence; Thierry, Jean-Claude; Kieffer, Bruno. "Letter to the Editor: 1H, 15N, 13C resonances of the N-terminal domain of the human TFIIH p62 subunit " J. Biomol. NMR 19, 281-282 (2001).

Assembly members:

Assembly members:
human TFIIH p62 subunit, polymer, 110 residues, 12432 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
human TFIIH p62 subunit: GSMATSSEEVLLIVKKVRQK KQDGALYLMAERIAWAPEGK DRFTISHMYADIKCQKISPE GKAKIQLQLVLHAGDTTNFH FSNESTAVKERDAVKDLLQQ LLPKFKRKAN

Data sets:

assigned_chemical_shifts
- shift_set_1

Data type	Count
1H chemical shifts	729
13C chemical shifts	316
15N chemical shifts	108

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	p62 monomer	1

Entities:

Entity 1, p62 monomer 110 residues - 12432 Da.

1	GLY	SER	MET	ALA	THR	SER	SER	GLU	GLU	VAL
2	LEU	LEU	ILE	VAL	LYS	LYS	VAL	ARG	GLN	LYS
3	LYS	GLN	ASP	GLY	ALA	LEU	TYR	LEU	MET	ALA
4	GLU	ARG	ILE	ALA	TRP	ALA	PRO	GLU	GLY	LYS
5	ASP	ARG	PHE	THR	ILE	SER	HIS	MET	TYR	ALA
6	ASP	ILE	LYS	CYS	GLN	LYS	ILE	SER	PRO	GLU
7	GLY	LYS	ALA	LYS	ILE	GLN	LEU	GLN	LEU	VAL
8	LEU	HIS	ALA	GLY	ASP	THR	THR	ASN	PHE	HIS
9	PHE	SER	ASN	GLU	SER	THR	ALA	VAL	LYS	GLU
10	ARG	ASP	ALA	VAL	LYS	ASP	LEU	LEU	GLN	GLN
11	LEU	LEU	PRO	LYS	PHE	LYS	ARG	LYS	ALA	ASN

Samples:

sample_1: human TFIIH p62 subunit, [13C; U-98% 15N], 1.5 mM

sample_2: human TFIIH p62 subunit 2.4 mM

cond_1: pH: 7.4; temperature: 293 K; pressure: 1 atm

Experiments:

Name	Sample	Sample state	Sample conditions
15N HSQC	not available	not available	cond_1
HNCA	not available	not available	cond_1
HN(CO)CA	not available	not available	cond_1
CBCA(CO)NH	not available	not available	cond_1
HNCACB	not available	not available	cond_1
15N-TOCSY-HSQC	not available	not available	cond_1
15N-NOESY-HSQC	not available	not available	cond_1
HBHA(CO)NH	not available	not available	cond_1
C(CO)NH	not available	not available	cond_1
H(CCO)NH	not available	not available	cond_1
HCCH-TOCSY	not available	not available	cond_1
2D NOESY	not available	not available	cond_1
2D TOCSY	not available	not available	cond_1

Software:

FELIX v95 - spectra processing

XEASY v1.3.10 - spectra assignment

NMR spectrometers:

Bruker DRX 600 MHz
Bruker DRX 800 MHz

PDB	1PFJ 2RNR 2RUK
DBJ	BAB15621 BAB23789 BAE02479 BAG10896 BAG35811
EMBL	CAA05340 CAG33649
GB	AAA58399 AAB23294 AAH00365 AAH04452 AAH52837
REF	NP_001039615 NP_001101955 NP_001135779 NP_001186521 NP_001271594
SP	P32780 Q9DBA9
TPG	DAA13968
AlphaFold	P32780 Q9DBA9

1	GLY	SER	MET	ALA	THR	SER	SER	GLU	GLU	VAL
2	LEU	LEU	ILE	VAL	LYS	LYS	VAL	ARG	GLN	LYS
3	LYS	GLN	ASP	GLY	ALA	LEU	TYR	LEU	MET	ALA
4	GLU	ARG	ILE	ALA	TRP	ALA	PRO	GLU	GLY	LYS
5	ASP	ARG	PHE	THR	ILE	SER	HIS	MET	TYR	ALA
6	ASP	ILE	LYS	CYS	GLN	LYS	ILE	SER	PRO	GLU
7	GLY	LYS	ALA	LYS	ILE	GLN	LEU	GLN	LEU	VAL
8	LEU	HIS	ALA	GLY	ASP	THR	THR	ASN	PHE	HIS
9	PHE	SER	ASN	GLU	SER	THR	ALA	VAL	LYS	GLU
10	ARG	ASP	ALA	VAL	LYS	ASP	LEU	LEU	GLN	GLN
11	LEU	LEU	PRO	LYS	PHE	LYS	ARG	LYS	ALA	ASN

1	GLY	SER	MET	ALA	THR	SER	SER	GLU	GLU	VAL
2	LEU	LEU	ILE	VAL	LYS	LYS	VAL	ARG	GLN	LYS
3	LYS	GLN	ASP	GLY	ALA	LEU	TYR	LEU	MET	ALA
4	GLU	ARG	ILE	ALA	TRP	ALA	PRO	GLU	GLY	LYS
5	ASP	ARG	PHE	THR	ILE	SER	HIS	MET	TYR	ALA
6	ASP	ILE	LYS	CYS	GLN	LYS	ILE	SER	PRO	GLU
7	GLY	LYS	ALA	LYS	ILE	GLN	LEU	GLN	LEU	VAL
8	LEU	HIS	ALA	GLY	ASP	THR	THR	ASN	PHE	HIS
9	PHE	SER	ASN	GLU	SER	THR	ALA	VAL	LYS	GLU
10	ARG	ASP	ALA	VAL	LYS	ASP	LEU	LEU	GLN	GLN
11	LEU	LEU	PRO	LYS	PHE	LYS	ARG	LYS	ALA	ASN