BMRB Entry 4729

Title:
1H, 15N, 13CA, 13CB, 13C' assignments of the EGF-like module pair 3-4 from vitamin K-dependent protein S
Deposition date:
2000-04-28
Original release date:
2000-05-11
Authors:
Muranyi, Andreas; Evenas, Johan; Stenberg, Yvonne; Stenflo, Johan; Drakenberg, Torbjorn
Citation:

Citation: Muranyi, Andreas; Evenas, Johan; Stenberg, Yvonne; Stenflo, Johan; Drakenberg, Torbjorn. "1H, 15N and 13C assignments and secondary structure of the EGF-like module pair 3-4 from vitamin K-dependent protein S"  FEBS Lett. ., .-..

Assembly members:

Assembly members:
EGF-like module pair 3-4 from human vitamin K-dependent protein S, polymer, 89 residues, Formula weight is not available
CA, non-polymer, 40.078 Da.

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
EGF-like module pair 3-4 from human vitamin K-dependent protein S: HMKDVDECSLKPSICGTAVC KNIPGDFECECPEGYRYNLK SKSCEDIDECSENMCAQLCV NYPGGYTCYCDGKKGFKLAQ DQKSCEVVS

Data sets:
Data typeCount
1H chemical shifts947
13C chemical shifts404
15N chemical shifts170

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1monomer 11
2monomer 21
3Ca 12
4Ca 22

Entities:

Entity 1, monomer 1 89 residues - Formula weight is not available

1   HISMETLYSASPVALASPGLUCYSSERLEU
2   LYSPROSERILECYSGLYTHRALAVALCYS
3   LYSASNILEPROGLYASPPHEGLUCYSGLU
4   CYSPROGLUGLYTYRARGTYRASNLEULYS
5   SERLYSSERCYSGLUASPILEASPGLUCYS
6   SERGLUASNMETCYSALAGLNLEUCYSVAL
7   ASNTYRPROGLYGLYTYRTHRCYSTYRCYS
8   ASPGLYLYSLYSGLYPHELYSLEUALAGLN
9   ASPGLNLYSSERCYSGLUVALVALSER

Entity 2, Ca 1 - Ca - 40.078 Da.

1   CA

Samples:

sample_1: EGF-like module pair 3-4 from human vitamin K-dependent protein S 1.5 mM

sample_2: EGF-like module pair 3-4 from human vitamin K-dependent protein S, [U-99% 15N], 0.5 – 1.5 mM

sample_3: EGF-like module pair 3-4 from human vitamin K-dependent protein S, [U-99% 15N; U-99% 13C], 1.2 – 1.9 mM

Ex-cond_1: pH: 6.0; temperature: 309 K

Experiments:

NameSampleSample stateSample conditions
1H NOESY, with mixing times 80 and 150 ms.not availablenot availableEx-cond_1
1H TOCSY, with DIPSI isotropic mixing times of 40 and 110 ms.not availablenot availableEx-cond_1
1H-15N HSQCnot availablenot availableEx-cond_1
1H-15N TOCSY-HSQC, with a DIPSI mixing sequence (40-110 ms)not availablenot availableEx-cond_1
1H-15N NOESY-HSQC, with mixing times of 80 and 150 msnot availablenot availableEx-cond_1
HNCAnot availablenot availableEx-cond_1
HN(CO)CAnot availablenot availableEx-cond_1
HNCACBnot availablenot availableEx-cond_1
HNCOnot availablenot availableEx-cond_1

Software:

FELIX v98.0 -

XEASY v1.3.13 -

NMR spectrometers:

  • Varain Unity-Inova 600 MHz

Related Database Links:

BMRB 17338 17462 4728
PDB
GB EAW68856
REF XP_009444184 XP_014199791

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks