BMRB Entry 4729

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4729

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 15N, 13CA, 13CB, 13C' assignments of the EGF-like module pair 3-4 from vitamin K-dependent protein S

Deposition date:

2000-04-28

Original release date:

2000-05-11

Authors:

Muranyi, Andreas; Evenas, Johan; Stenberg, Yvonne; Stenflo, Johan; Drakenberg, Torbjorn

Citation:

Citation: Muranyi, Andreas; Evenas, Johan; Stenberg, Yvonne; Stenflo, Johan; Drakenberg, Torbjorn. "1H, 15N and 13C assignments and secondary structure of the EGF-like module pair 3-4 from vitamin K-dependent protein S" FEBS Lett. ., .-..

Assembly members:

Assembly members:
EGF-like module pair 3-4 from human vitamin K-dependent protein S, polymer, 89 residues, Formula weight is not available
CA, non-polymer, 40.078 Da.

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
EGF-like module pair 3-4 from human vitamin K-dependent protein S: HMKDVDECSLKPSICGTAVC KNIPGDFECECPEGYRYNLK SKSCEDIDECSENMCAQLCV NYPGGYTCYCDGKKGFKLAQ DQKSCEVVS

Data sets:

assigned_chemical_shifts
- pS_EGF_3-4_major_form
- pS_EGF_3-4_minor_form

Data type	Count
1H chemical shifts	947
13C chemical shifts	404
15N chemical shifts	170

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	monomer 1	1
2	monomer 2	1
3	Ca 1	2
4	Ca 2	2

Entities:

Entity 1, monomer 1 89 residues - Formula weight is not available

1

HIS

MET

LYS

ASP

VAL

ASP

GLU

CYS

SER

LEU

2

LYS

PRO

SER

ILE

CYS

GLY

THR

ALA

VAL

CYS

3

LYS

ASN

ILE

PRO

GLY

ASP

PHE

GLU

CYS

GLU

4

CYS

PRO

GLU

GLY

TYR

ARG

TYR

ASN

LEU

LYS

5

SER

LYS

SER

CYS

GLU

ASP

ILE

ASP

GLU

CYS

6

SER

GLU

ASN

MET

CYS

ALA

GLN

LEU

CYS

VAL

7

ASN

TYR

PRO

GLY

TYR

THR

CYS

TYR

CYS

8

ASP

GLY

LYS

GLY

PHE

LYS

LEU

ALA

GLN

9

ASP

GLN

LYS

SER

CYS

GLU

VAL

SER

Entity 2, Ca 1 - Ca - 40.078 Da.

1

CA

Samples:

sample_1: EGF-like module pair 3-4 from human vitamin K-dependent protein S 1.5 mM

sample_2: EGF-like module pair 3-4 from human vitamin K-dependent protein S, [U-99% 15N], 0.5 – 1.5 mM

sample_3: EGF-like module pair 3-4 from human vitamin K-dependent protein S, [U-99% 15N; U-99% 13C], 1.2 – 1.9 mM

Ex-cond_1: pH: 6.0; temperature: 309 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H NOESY, with mixing times 80 and 150 ms.	not available	not available	Ex-cond_1
1H TOCSY, with DIPSI isotropic mixing times of 40 and 110 ms.	not available	not available	Ex-cond_1
1H-15N HSQC	not available	not available	Ex-cond_1
1H-15N TOCSY-HSQC, with a DIPSI mixing sequence (40-110 ms)	not available	not available	Ex-cond_1
1H-15N NOESY-HSQC, with mixing times of 80 and 150 ms	not available	not available	Ex-cond_1
HNCA	not available	not available	Ex-cond_1
HN(CO)CA	not available	not available	Ex-cond_1
HNCACB	not available	not available	Ex-cond_1
HNCO	not available	not available	Ex-cond_1

Software:

FELIX v98.0 -

XEASY v1.3.13 -

NMR spectrometers:

Varain Unity-Inova 600 MHz

BMRB	17338 17462 4728
PDB	1Z6C
GB	EAW68856
REF	XP_009444184 XP_014199791