BMRB Entry 17462

Title:
EGF-like Module Pair 3-4 in Vitamin K-dependent Protein S: Modulation of Calcium Affinity of Module 4 by Module 3, and Interaction with Factor X
Deposition date:
2011-02-11
Original release date:
2012-06-06
Authors:
Stenberg, Yvonne; Muranyi, Andreas; Steen, Christina; Thulin, Eva; Drakenberg, Torbjorn; Stenflo, Johan
Citation:

Citation: Stenberg, Yvonne; Muranyi, Andreas; Steen, Christina; Thulin, Eva; Drakenberg, Torbjorn; Stenflo, Johan. "EGF-like Module Pair 3-4 in Vitamin K-dependent Protein S: Modulation of Calcium Affinity of Module 4 by Module 3, and Interaction with Factor X"  J. Mol. Biol. 293, 653-665 (1999).

Assembly members:

Assembly members:
EGF, polymer, 109 residues, 40.078 Da.
CA, non-polymer, 40.078 Da.

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET16b

Data sets:
  • binding_constants
  • pKa_values
Data typeCount
binding constants2
pKa values1

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1EGF1
2CA2

Entities:

Entity 1, EGF 109 residues - 40.078 Da.

1   METGLYHISHISHISHISHISHISHISHIS
2   HISHISSERSERGLYHISILEGLUGLYARG
3   HISMETLYSASPVALASPGLUCYSSERLEU
4   LYSPROSERILECYSGLYTHRALAVALCYS
5   LYSASNILEPROGLYASPPHEGLUCYSGLU
6   CYSPROGLUGLYTYRARGTYRASNLEULYS
7   SERLYSSERCYSGLUASPILEASPGLUCYS
8   SERGLUASNMETCYSALAGLNLEUCYSVAL
9   ASNTYRPROGLYGLYTYRTHRCYSTYRCYS
10   ASPGLYLYSLYSGLYPHELYSLEUALAGLN
11   ASPGLNLYSSERCYSGLUVALVALSER

Entity 2, CA - Ca - 40.078 Da.

1   CA

Samples:

sample_1: EGF, [U-15N], 0.214 mM; sodium chloride 150 mM; sodium chloride 0.1 mM; DSS 1 mM; calcium chloride0.040 – 0.200 M; H2O 90%; D2O 10%

sample_conditions_1: pH: 7.5; pressure: 1 atm; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

FELIX v97, Accelrys Software Inc. - processing

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

BMRB 17338 4729
PDB
REF XP_009444184 XP_014199791