BMRB Entry 17338

Name: EGF-like Module Pair 3-4 in Vitamin K-dependent Protein S: Modulation of Calcium Affinity of Module 4 by Module 3, and Interaction with Factor X
Published: 2012-06-06

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17338

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

EGF-like Module Pair 3-4 in Vitamin K-dependent Protein S: Modulation of Calcium Affinity of Module 4 by Module 3, and Interaction with Factor X

Deposition date:

2010-12-02

Original release date:

2012-06-06

Authors:

Stenberg, Yvonne; Muranyi, Andreas; Steen, Christina; Thulin, Eva; Drakenberg, Torbjorn; Stenflo, Johan

Citation:

Citation: Stenberg, Yvonne; Muranyi, Andreas; Steen, Christina; Thulin, Eva; Drakenberg, Torbjorn. "EGF-like Module Pair 3-4 in Vitamin K-dependent Protein S: Modulation of Calcium Affinity of Module 4 by Module 3 and Interaction with Factor X" J. Mol. Biol. 293, 653-665 (1999).

Assembly members:

Assembly members:
EGF34, polymer, 109 residues, Formula weight is not available
CA, non-polymer, 40.078 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET 16b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
EGF34: MGHHHHHHHHHHSSGHIEGR HMKDVDECSLKPSICGTAVC KNIPGDFECECPEGYRYNLK SKSCEDIDECSENMCAQLCV NYPGGYTCYCDGKKGFKLAQ DQKSCEVVS

Data sets:

binding_constants

Data type	Count
binding constants	3

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	EGF34	1
2	CA	2

Entities:

Entity 1, EGF34 109 residues - Formula weight is not available

1

MET

GLY

HIS

2

HIS

SER

GLY

HIS

ILE

GLU

GLY

ARG

3

HIS

MET

LYS

ASP

VAL

ASP

GLU

CYS

SER

LEU

4

LYS

PRO

SER

ILE

CYS

GLY

THR

ALA

VAL

CYS

5

LYS

ASN

ILE

PRO

GLY

ASP

PHE

GLU

CYS

GLU

6

CYS

PRO

GLU

GLY

TYR

ARG

TYR

ASN

LEU

LYS

7

SER

LYS

SER

CYS

GLU

ASP

ILE

ASP

GLU

CYS

8

SER

GLU

ASN

MET

CYS

ALA

GLN

LEU

CYS

VAL

9

ASN

TYR

PRO

GLY

TYR

THR

CYS

TYR

CYS

10

ASP

GLY

LYS

GLY

PHE

LYS

LEU

ALA

GLN

11

ASP

GLN

LYS

SER

CYS

GLU

VAL

SER

Entity 2, CA - Ca - 40.078 Da.

1

CA

Samples:

sample_1: EGF34, [U-15N], 0.214 mM; sodium chloride 150 mM; sodium azide 0.1 mM; DSS 1 mM; calcium chloride0.040 – 0.200 M; H2O 90%; D2O 10%

sample_conditions_1: pH: 7.5; pressure: 1 atm; temperature: 300 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1

Software:

FELIX v97, Accelrys Software Inc. - processing

NMR spectrometers:

Varian INOVA 600 MHz

BMRB	17462 4729
PDB	1Z6C
REF	XP_009444184 XP_014199791