BMRB Entry 4709

Name: WT1+KTS/free
Published: 2000-05-01

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4709

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

WT1+KTS/free

Deposition date:

2000-03-30

Original release date:

2000-05-01

Authors:

Laity, John; Chung, John; Dyson, H.; Wright, Peter

Citation:

Citation: Laity, John; Chung, John; Dyson, H.; Wright, Peter. "Alternative Splicing of Wilms' Tumor Suppressor Protein Modulates DNA Binding Activity Through Isoform-Specific DNA-Induced Conformational Changes" Biochemistry ., .-. (2000).

Assembly members:

Assembly members:
WT1, polymer, 122 residues, 14766 Da.
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: not available Genus/species: Homo Sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: PET21

Entity Sequences (FASTA):

Entity Sequences (FASTA):
WT1: ASEKRPFMCAYPGCNKRYFK LSHLQMHSRKHTGEKPYQCD FKDCERRFSRSDQLKRHQRR HTGVKPFQCKTCQRKFSRSD HLKTHTRTHTGKTSEKPFSC RWPSCQKKFARSDELVRHHN MH

Data sets:

assigned_chemical_shifts
- chemical_shift_assignment_one

Data type	Count
13C chemical shifts	353
15N chemical shifts	113
1H chemical shifts	113

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	WT1+KTS	1
2	Zn 1	2
3	Zn 2	2
4	Zn 3	2
5	Zn 4	2

Entities:

Entity 1, WT1+KTS 122 residues - 14766 Da.

1

ALA

SER

GLU

LYS

ARG

PRO

PHE

MET

CYS

ALA

2

TYR

PRO

GLY

CYS

ASN

LYS

ARG

TYR

PHE

LYS

3

LEU

SER

HIS

LEU

GLN

MET

HIS

SER

ARG

LYS

4

HIS

THR

GLY

GLU

LYS

PRO

TYR

GLN

CYS

ASP

5

PHE

LYS

ASP

CYS

GLU

ARG

PHE

SER

ARG

6

SER

ASP

GLN

LEU

LYS

ARG

HIS

GLN

ARG

7

HIS

THR

GLY

VAL

LYS

PRO

PHE

GLN

CYS

LYS

8

THR

CYS

GLN

ARG

LYS

PHE

SER

ARG

SER

ASP

9

HIS

LEU

LYS

THR

HIS

THR

ARG

THR

HIS

THR

10

GLY

LYS

THR

SER

GLU

LYS

PRO

PHE

SER

CYS

11

ARG

TRP

PRO

SER

CYS

GLN

LYS

PHE

ALA

12

ARG

SER

ASP

GLU

LEU

VAL

ARG

HIS

ASN

13

MET

HIS

Entity 2, Zn 1 - Zn - 65.409 Da.

1

ZN

Samples:

free_proteins: WT1, [U-95% 13C; U-95% 15N], 1.0 – 1.5 mM

Conditions: pH: 6.70; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
15N-HSQC	free_proteins	not available	Conditions
HNCA	free_proteins	not available	Conditions
HN(CO)CA	free_proteins	not available	Conditions
HNCACB	free_proteins	not available	Conditions
CBCA(CO)NH	free_proteins	not available	Conditions
HNCO	free_proteins	not available	Conditions
HCACO(CA)NH	free_proteins	not available	Conditions
HNCACB (deuterium decoupling)	free_proteins	not available	Conditions
HN(CO)CACB (deuterium decoupling)	free_proteins	not available	Conditions

Software:

FELIX v95, 97 - processing, peakpicking, analysis

NMR spectrometers:

Bruker AMX 500 MHz

BMRB	15532 15533 4707 4708 4710
PDB	2JP9 2JPA 2PRT
DBJ	BAA28147 BAA76399 BAA94794 BAF84425 BAG52667
EMBL	CAA35956 CAA49373 CAA59735 CAA59737 CDG23662
GB	AAA40573 AAA61299 AAB27319 AAB33427 AAB70832
PRF	1604420A
REF	NP_000369 NP_001001264 NP_001185481 NP_077742 NP_077744
SP	B7ZSG3 O62651 P19544 P22561 P49952
TPG	DAA21902
AlphaFold	O62651 B7ZSG3 P19544 P49952 P22561