BMRB Entry 15532

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PDB ID: 2jp9
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR15532
MolProbity Validation Chart

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Title:
Structure of the Wilms Tumor Suppressor Protein Zinc Finger Domain Bound to DNA
Deposition date:
2007-10-18
Original release date:
2008-02-14
Authors:
Stoll, Raphael; Lee, Brian; Debler, Erik; Laity, John; Wilson, Ian; Dyson, Helen; Wright, Peter
Citation:

Citation: Stoll, Raphael; Lee, Brian; Debler, Erik; Laity, John; Wilson, Ian; Dyson, Helen; Wright, Peter. "Structure of the Wilms' Tumor Suppressor Protein Zinc Finger Domain Bound to DNA"  J. Mol. Biol. 372, 1227-1245 (2007).
PubMed: 17716689

Assembly members:

Assembly members:
wt1-17mer, polymer, 119 residues, 14494.849 Da.
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET21d

Entity Sequences (FASTA):

Entity Sequences (FASTA):
wt1-17mer: ASEKRPFMCAYPGCNKRYFK LSHLQMHSRKHTGEKPYQCD FKDCERRFSRSDQLKRHQRR HTGVKPFQCKTCQRKFSRSD HLKTHTRTHTGEKPFSCRWP SCQKKFARSDELVRHHNMH

Data sets:
Data typeCount
15N chemical shifts110
1H chemical shifts110

Additional metadata:

  • Assembly
  • Samples and Experiments
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Assembly:

Entity Assembly IDEntity NameEntity ID
1wt1-17mer1
2zn12
3zn22
4zn32
5zn42

Entities:

Entity 1, wt1-17mer 119 residues - 14494.849 Da.

1   ALASERGLULYSARGPROPHEMETCYSALA
2   TYRPROGLYCYSASNLYSARGTYRPHELYS
3   LEUSERHISLEUGLNMETHISSERARGLYS
4   HISTHRGLYGLULYSPROTYRGLNCYSASP
5   PHELYSASPCYSGLUARGARGPHESERARG
6   SERASPGLNLEULYSARGHISGLNARGARG
7   HISTHRGLYVALLYSPROPHEGLNCYSLYS
8   THRCYSGLNARGLYSPHESERARGSERASP
9   HISLEULYSTHRHISTHRARGTHRHISTHR
10   GLYGLULYSPROPHESERCYSARGTRPPRO
11   SERCYSGLNLYSLYSPHEALAARGSERASP
12   GLULEUVALARGHISHISASNMETHIS

Entity 2, zn1 - Zn - 65.409 Da.

1   ZN

Related Database Links:

BMRB 15533 4707 4708 4709 4710
PDB 2JP9 2JPA 2PRT
DBJ BAA11522 BAA28147 BAA94793 BAF84425
EMBL CAA35956 CAA43819 CDG23662
GB AAA36810 AAA61299 AAB33443 AAB53152 AAH32861
PRF 1604420A
REF NP_000369 NP_001001264 NP_001079057 NP_001079336 NP_001135625
SP B5DE03 O62651 P19544 P79958
TPG DAA21902
AlphaFold B5DE03 O62651 P19544 P79958

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