BMRB Entry 4708

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4708

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Title:
WT1-KTS/DNA complex
Deposition date:
2000-03-30
Original release date:
2000-05-01
Authors:
Laity, John; Chung, John; Dyson, H.; Wright, Peter
Citation:

Citation: Laity, John; Chung, John; Dyson, H.; Wright, Peter. "Alternative Splicing of Wilms' Tumor Suppressor Protein Modulates DNA Binding Activity Through Isoform-Specific DNA-Induced Conformational Changes"  Biochemistry ., .-. (2000).

Assembly members:

Assembly members:
WT1, polymer, 119 residues, 14450 Da.
DNA strand 1, polymer, 14 residues, Formula weight is not available
DNA strand 2, polymer, 14 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Homo Sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: PET21

Entity Sequences (FASTA):

Entity Sequences (FASTA):
WT1: ASEKRPFMCAYPGCNKRYFK LSHLQMHSRKHTGEKPYQCD FKDCERRFSRSDQLKRHQRR HTGVKPFQCKTCQRKFSRSD HLKTHTRTHTGEKPFSCRWP SCQKKFARSDELVRHHNMH
DNA strand 1: CGCGGGGGCGTCTG
DNA strand 2: CAGACGCCCCCGCG

Data sets:
Data typeCount
13C chemical shifts227
1H chemical shifts108
15N chemical shifts108

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1WT1-KTS1
2DNA strand 12
3DNA strand 23
4Zn 14
5Zn 24
6Zn 34
7Zn 44

Entities:

Entity 1, WT1-KTS 119 residues - 14450 Da.

1   ALASERGLULYSARGPROPHEMETCYSALA
2   TYRPROGLYCYSASNLYSARGTYRPHELYS
3   LEUSERHISLEUGLNMETHISSERARGLYS
4   HISTHRGLYGLULYSPROTYRGLNCYSASP
5   PHELYSASPCYSGLUARGARGPHESERARG
6   SERASPGLNLEULYSARGHISGLNARGARG
7   HISTHRGLYVALLYSPROPHEGLNCYSLYS
8   THRCYSGLNARGLYSPHESERARGSERASP
9   HISLEULYSTHRHISTHRARGTHRHISTHR
10   GLYGLULYSPROPHESERCYSARGTRPPRO
11   SERCYSGLNLYSLYSPHEALAARGSERASP
12   GLULEUVALARGHISHISASNMETHIS

Entity 2, DNA strand 1 14 residues - Formula weight is not available

1   DCDGDCDGDGDGDGDGDCDG
2   DTDCDTDG

Entity 3, DNA strand 2 14 residues - Formula weight is not available

1   DCDADGDADCDGDCDCDCDC
2   DCDGDCDG

Entity 4, Zn 1 - Zn - 65.409 Da.

1   ZN

Samples:

WT1-DNA_complex: WT1, [U-95% 13C; U-95% 15N], 0.15 – 0.3 mM; DNA strand 10.15 – 0.3 mM; DNA strand 20.15 – 0.3 mM

Conditions: pH: 6.70; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
15N-HSQCWT1-DNA_complexnot availableConditions
HNCAWT1-DNA_complexnot availableConditions
HN(CO)CAWT1-DNA_complexnot availableConditions
HNCACBWT1-DNA_complexnot availableConditions
CBCA(CO)NHWT1-DNA_complexnot availableConditions
HNCOWT1-DNA_complexnot availableConditions
HCACO(CA)NHWT1-DNA_complexnot availableConditions
HNCACB (deuterium decoupling)WT1-DNA_complexnot availableConditions
HN(CO)CACB (deuterium decoupling)WT1-DNA_complexnot availableConditions

Software:

FELIX v95, 97 - processing, peakpicking, analysis

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

BMRB 15532 15533 4707 4709 4710
PDB
DBJ BAA11522 BAA28147 BAA94793 BAF84425
EMBL CAA35956 CAA43819 CDG23662
GB AAA36810 AAA61299 AAB33443 AAB53152 AAH32861
PRF 1604420A
REF NP_000369 NP_001001264 NP_001079057 NP_001079336 NP_001135625
SP B5DE03 O62651 P19544 P79958
TPG DAA21902
AlphaFold B5DE03 O62651 P19544 P79958

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks