BMRB Entry 4695

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR4695

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
Effect of the single mutation His 64->Phe on the stability and folding of apomyoglobin
Deposition date:
2000-03-20
Original release date:
2001-10-09
Authors:
Garcia, Carlos; Cavagnero, Silvia; Nishimura, Chiaki; Dyson, H.; Wright, Peter
Citation:

Citation: Garcia, Carlos; Nishimura, Chiaki; Cavagnero, Silvia; Dyson, H.; Wright, Peter. "Changes in the apomyoglobin folding pathway caused by mutation of the distal histidine residue"  Biochemistry 39, 11227-11237 (2000).
PubMed: 10985768

Assembly members:

Assembly members:
myoglobin, polymer, 153 residues, Formula weight is not available
HEA, non-polymer, 852.837 Da.

Natural source:

Natural source:   Common Name: sperm whale   Taxonomy ID: 9755   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Physeter catodon

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
myoglobin: VLSEGEWQLVLHVWAKVEAD VAGHGQDILIRLFKSHPETL EKFDRFKHLKTEAEMKASED LKKFGVTVLTALGAILKKKG HHEAELKPLAQSHATKHKIP IKYLEFISEAIIHVLHSRHP GDFGADAQGAMNKALELFRK DIAAKYKELGYQG

Data sets:
Data typeCount
13C chemical shifts282
15N chemical shifts147
1H chemical shifts147

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1myoglobin H64F mutant1

Entities:

Entity 1, myoglobin H64F mutant 153 residues - Formula weight is not available

1   VALLEUSERGLUGLYGLUTRPGLNLEUVAL
2   LEUHISVALTRPALALYSVALGLUALAASP
3   VALALAGLYHISGLYGLNASPILELEUILE
4   ARGLEUPHELYSSERHISPROGLUTHRLEU
5   GLULYSPHEASPARGPHELYSHISLEULYS
6   THRGLUALAGLUMETLYSALASERGLUASP
7   LEULYSLYSPHEGLYVALTHRVALLEUTHR
8   ALALEUGLYALAILELEULYSLYSLYSGLY
9   HISHISGLUALAGLULEULYSPROLEUALA
10   GLNSERHISALATHRLYSHISLYSILEPRO
11   ILELYSTYRLEUGLUPHEILESERGLUALA
12   ILEILEHISVALLEUHISSERARGHISPRO
13   GLYASPPHEGLYALAASPALAGLNGLYALA
14   METASNLYSALALEUGLULEUPHEARGLYS
15   ASPILEALAALALYSTYRLYSGLULEUGLY
16   TYRGLNGLY

Samples:

sample_1: myoglobin, [U-95% 13C; U-90% 15N], 1 mM

ex-cond_1: pH: 5.8; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
HNCAsample_1not availableex-cond_1
CBCA(CO)NHsample_1not availableex-cond_1

Software:

FELIX v97 - process and plot spectra

NMRView - process and plot

NMR spectrometers:

  • Bruker AMX 500 MHz

Related Database Links:

BMRB 1027 1029 1200 1413 1455 1457 1459 1461 1463 1465 1467 1469 1471 15589 16217 16218 16499 16500 16501 1752 2345 2346 2347 2348 2431 2432 2433 2434 291 292 293 40 4061 4062 426 4568 4676
PDB
DBJ BAF03579
GB AAA72199
PRF 742482A
REF NP_001277651
SP P02185
AlphaFold P02185

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks