BMRB Entry 4061

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4061

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Title:
Overexpression of Myoglobin and Assignment of Its Amide, C Alpha and C Beta Resonances
Deposition date:
1997-10-02
Original release date:
1998-02-25
Authors:
Jennings, Patricia; Stone, Martin; Wright, Peter
Citation:

Citation: Jennings, Patricia; Stone, Martin; Wright, Peter. "Overexpression of Myoglobin and Assignment of Its Amide, C Alpha and C Beta Resonances"  J. Biomol. NMR 6, 271-276 (1995).

Assembly members:

Assembly members:
apomyoglobin, polymer, 154 residues, Formula weight is not available
PROTOPORPHYRIN IX, non-polymer, 562.658 Da.
CARBON MONOXIDE, non-polymer, 28.010 Da.

Natural source:

Natural source:   Common Name: sperm whale   Taxonomy ID: 9755   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Physeter catodon

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pCKR102

Entity Sequences (FASTA):

Entity Sequences (FASTA):
apomyoglobin: MVLSEGEWQLVLHVWAKVEA DVAGHGQDILIRLFKSHPET LEKFDRFKHLKTEAEMKASE DLKKHGVTVLTALGAILKKK GHHEAELKPLAQSHATKHKI PIKYLEFISEAIIHVLHSRH PGDFGADAQGAMNKALELFR KDIAAKYKELGYQG

Data sets:
Data typeCount
13C chemical shifts267
15N chemical shifts143
1H chemical shifts143

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1apomyoglobin1
2Iron protoporphyrin IX2
3Carbon monoxide3

Entities:

Entity 1, apomyoglobin 154 residues - Formula weight is not available

1   METVALLEUSERGLUGLYGLUTRPGLNLEU
2   VALLEUHISVALTRPALALYSVALGLUALA
3   ASPVALALAGLYHISGLYGLNASPILELEU
4   ILEARGLEUPHELYSSERHISPROGLUTHR
5   LEUGLULYSPHEASPARGPHELYSHISLEU
6   LYSTHRGLUALAGLUMETLYSALASERGLU
7   ASPLEULYSLYSHISGLYVALTHRVALLEU
8   THRALALEUGLYALAILELEULYSLYSLYS
9   GLYHISHISGLUALAGLULEULYSPROLEU
10   ALAGLNSERHISALATHRLYSHISLYSILE
11   PROILELYSTYRLEUGLUPHEILESERGLU
12   ALAILEILEHISVALLEUHISSERARGHIS
13   PROGLYASPPHEGLYALAASPALAGLNGLY
14   ALAMETASNLYSALALEUGLULEUPHEARG
15   LYSASPILEALAALALYSTYRLYSGLULEU
16   GLYTYRGLNGLY

Entity 2, Iron protoporphyrin IX - C34 H34 N4 O4 - 562.658 Da.

1   PP9

Entity 3, Carbon monoxide - C O - 28.010 Da.

1   CMO

Samples:

sample_one: myoglobin carbon monoxide complex, [U-90% 15N; U-95% 13C], 1 mM; potassium phosphate 0.05 M; H2O 90%; D2O 10%

experimental_conditions_MbCO: pH: 5.6; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
not availablesample_onenot availableexperimental_conditions_MbCO

Software:

FELIX v2.05, Hare Research -

NMR spectrometers:

  • Bruker AMX500 500 MHz

Related Database Links:

BMRB 1027 1029 1200 1413 1455 1457 1459 1461 1463 1465 1467 1469 1471 15589 16217 16218 16499 16500 16501 1752 2345 2346 2347 2348 2431 2432 2433 2434 291 292 293 40 4062 426 4568 4676 4695
PDB
DBJ BAF03579
GB AAA72199
PRF 742482A
REF NP_001277651
SP P02185
AlphaFold P02185

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