BMRB Entry 4676
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4676
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Yao, Jian; Chung, J.; Eliezer, D.; Wright, Peter; Dyson, H.. "NMR Structural and Dynamic Characterization of the Acid-unfolded State of
Apomyoglobin Provides Insights into the Early Events in Protein Folding" Biochemistry 40, 3561-3571 (2001).
PubMed: 11297422
Assembly members:
unfolded myoglobin, polymer, 153 residues, Formula weight is not available
Natural source: Common Name: sperm whale Taxonomy ID: 9755 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Physeter catodon
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
unfolded myoglobin: VLSEGEWQLVLHVWAKVEAD
VAGHGQDILIRLFKSHPETL
EKFDRFKHLKTEAEMKASED
LKKHGVTVLTALGAILKKKG
HHEAELKPLAQSHATKHKIP
IKYLEFISEAIIHVLHSRHP
GDFGADAQGAMNKALELFRK
DIAAKYKELGYQG
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 294 |
| 13C chemical shifts | 445 |
| 15N chemical shifts | 147 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | apoMb | 1 |
Entities:
Entity 1, apoMb 153 residues - Formula weight is not available
| 1 | VAL | LEU | SER | GLU | GLY | GLU | TRP | GLN | LEU | VAL | ||||
| 2 | LEU | HIS | VAL | TRP | ALA | LYS | VAL | GLU | ALA | ASP | ||||
| 3 | VAL | ALA | GLY | HIS | GLY | GLN | ASP | ILE | LEU | ILE | ||||
| 4 | ARG | LEU | PHE | LYS | SER | HIS | PRO | GLU | THR | LEU | ||||
| 5 | GLU | LYS | PHE | ASP | ARG | PHE | LYS | HIS | LEU | LYS | ||||
| 6 | THR | GLU | ALA | GLU | MET | LYS | ALA | SER | GLU | ASP | ||||
| 7 | LEU | LYS | LYS | HIS | GLY | VAL | THR | VAL | LEU | THR | ||||
| 8 | ALA | LEU | GLY | ALA | ILE | LEU | LYS | LYS | LYS | GLY | ||||
| 9 | HIS | HIS | GLU | ALA | GLU | LEU | LYS | PRO | LEU | ALA | ||||
| 10 | GLN | SER | HIS | ALA | THR | LYS | HIS | LYS | ILE | PRO | ||||
| 11 | ILE | LYS | TYR | LEU | GLU | PHE | ILE | SER | GLU | ALA | ||||
| 12 | ILE | ILE | HIS | VAL | LEU | HIS | SER | ARG | HIS | PRO | ||||
| 13 | GLY | ASP | PHE | GLY | ALA | ASP | ALA | GLN | GLY | ALA | ||||
| 14 | MET | ASN | LYS | ALA | LEU | GLU | LEU | PHE | ARG | LYS | ||||
| 15 | ASP | ILE | ALA | ALA | LYS | TYR | LYS | GLU | LEU | GLY | ||||
| 16 | TYR | GLN | GLY |
Samples:
sample_1: unfolded myoglobin, [U-95% 13C; U-95% 15N], 0.2 mM
Ex-cond-1: pH: 2.3; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| high field was required for maximum sensitivity for low concentration samples and for | sample_1 | not available | Ex-cond-1 |
| maximum proton dispersion | sample_1 | not available | Ex-cond-1 |
Software:
FELIX v97 - transform and plot; data tables assignments were done by hand
NMR spectrometers:
- Bruker DMX 750 MHz
Related Database Links:
| BMRB | 1027 1029 1200 1413 1455 1457 1459 1461 1463 1465 1467 1469 1471 15589 16217 16218 16499 16500 16501 1752 2345 2346 2347 2348 2431 2432 2433 2434 291 292 293 40 4061 4062 426 4568 4695 |
| PDB | |
| DBJ | BAF03579 |
| GB | AAA72199 |
| PRF | 742482A |
| REF | NP_001277651 |
| SP | P02185 |
| AlphaFold | P02185 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks