BMRB Entry 4681
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4681
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Lu, Jianyun; Lin, Chan-Lan; Tang, Changguo; Ponder, Jay; Kao, Jeff; Cistola, David; Li, Ellen. "The structure and dynamics of rat apo-cellular retinol-binding protein II in
solution: comparison with the X-ray structure" J. Mol. Biol. 286, 1179-1195 (1999).
PubMed: 10047490
Assembly members:
Apo cellular retinol-binding protein II, polymer, 134 residues, Formula weight is not available
Natural source: Common Name: norway rat Taxonomy ID: 10116 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rattus norvegicus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pMON-CRBPII
Entity Sequences (FASTA):
Apo cellular retinol-binding protein II: MTKDQNGTWEMESNENFEGY
MKALDIDFATRKIAVRLTQT
KIIVQDGDNFKTKTNSTFRN
YDLDFTVGVEFDEHTKGLDG
RNVKTLVTWEGNTLVCVQKG
EKENRGWKQWVEGDKLYLEL
TCGDQVCRQVFKKK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 548 |
| 1H chemical shifts | 1667 |
| 15N chemical shifts | 328 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Apo CRBP II | 1 |
Entities:
Entity 1, Apo CRBP II 134 residues - Formula weight is not available
| 1 | MET | THR | LYS | ASP | GLN | ASN | GLY | THR | TRP | GLU | ||||
| 2 | MET | GLU | SER | ASN | GLU | ASN | PHE | GLU | GLY | TYR | ||||
| 3 | MET | LYS | ALA | LEU | ASP | ILE | ASP | PHE | ALA | THR | ||||
| 4 | ARG | LYS | ILE | ALA | VAL | ARG | LEU | THR | GLN | THR | ||||
| 5 | LYS | ILE | ILE | VAL | GLN | ASP | GLY | ASP | ASN | PHE | ||||
| 6 | LYS | THR | LYS | THR | ASN | SER | THR | PHE | ARG | ASN | ||||
| 7 | TYR | ASP | LEU | ASP | PHE | THR | VAL | GLY | VAL | GLU | ||||
| 8 | PHE | ASP | GLU | HIS | THR | LYS | GLY | LEU | ASP | GLY | ||||
| 9 | ARG | ASN | VAL | LYS | THR | LEU | VAL | THR | TRP | GLU | ||||
| 10 | GLY | ASN | THR | LEU | VAL | CYS | VAL | GLN | LYS | GLY | ||||
| 11 | GLU | LYS | GLU | ASN | ARG | GLY | TRP | LYS | GLN | TRP | ||||
| 12 | VAL | GLU | GLY | ASP | LYS | LEU | TYR | LEU | GLU | LEU | ||||
| 13 | THR | CYS | GLY | ASP | GLN | VAL | CYS | ARG | GLN | VAL | ||||
| 14 | PHE | LYS | LYS | LYS |
Samples:
sample_1: Apo cellular retinol-binding protein II, [U-99% 13C; U-99% 15N], 1.0 – 2.0 mM
sample_2: Apo cellular retinol-binding protein II 1.5 mM
sample_3: Apo cellular retinol-binding protein II, [U-99% 15N], 1.5 mM
Ex-con_1: pH: 7.4; temperature: 298 K; ionic strength: 0.081 M
Ex-con_2: pH: 6.5; temperature: 298 K; ionic strength: 0.079 M
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|
Software:
No software information available
NMR spectrometers:
- Varian Unity 500 MHz
- Varian Unity 600 MHz
Related Database Links:
| BMRB | 4682 |
| PDB | |
| DBJ | BAB22708 |
| EMBL | CAA52268 |
| GB | AAA42022 AAI25552 AAI25554 EDL20985 EDL77463 |
| REF | NP_033060 NP_036772 XP_006243669 |
| SP | P06768 Q08652 |
| AlphaFold | P06768 Q08652 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks