BMRB Entry 4682

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PDB ID: 1eii
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4682
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone and side-chain 1H, 13C, and 15N Chemical Shift Assignments for holo-CRBP II

Deposition date:

2000-03-06

Original release date:

2000-08-03

Authors:

Lu, Jianyun; Lin, Chan-Lan; Tang, Changguo; Ponder, Jay; Kao, Jeff; Cistola, David; Li, Ellen

Citation:

Citation: Lu, Jianyun; Lin, Chan-Lan; Tang, Changguo; Ponder, Jay; Kao, Jeff; Cistola, David; Li, Ellen. "Binding of retinol induces changes in rat cellular retinol-binding protein II conformation and backbone dynamics" J. Mol. Biol. 300, 619-632 (2000).
PubMed: 10884357

Assembly members:

Assembly members:
Holo cellular retinol-binding protein II, polymer, 134 residues, 286.452 Da.
RTL, non-polymer, 286.452 Da.

Natural source:

Natural source: Common Name: norway rat Taxonomy ID: 10116 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rattus norvegicus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pMON-CRBPII

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Holo cellular retinol-binding protein II: MTKDQNGTWEMESNENFEGY MKALDIDFATRKIAVRLTQT KIIVQDGDNFKTKTNSTFRN YDLDFTVGVEFDEHTKGLDG RNVKTLVTWEGNTLVCVQKG EKENRGWKQWVEGDKLYLEL TCGDQVCRQVFKKK

Data sets:

assigned_chemical_shifts

Data type	Count
13C chemical shifts	544
1H chemical shifts	1496
15N chemical shifts	304

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Holo CRBP II	1
2	RTL	2

Entities:

Entity 1, Holo CRBP II 134 residues - 286.452 Da.

1

MET

THR

LYS

ASP

GLN

ASN

GLY

THR

TRP

GLU

2

MET

GLU

SER

ASN

GLU

ASN

PHE

GLU

GLY

TYR

3

MET

LYS

ALA

LEU

ASP

ILE

ASP

PHE

ALA

THR

4

ARG

LYS

ILE

ALA

VAL

ARG

LEU

THR

GLN

THR

5

LYS

ILE

VAL

GLN

ASP

GLY

ASP

ASN

PHE

6

LYS

THR

LYS

THR

ASN

SER

THR

PHE

ARG

ASN

7

TYR

ASP

LEU

ASP

PHE

THR

VAL

GLY

VAL

GLU

8

PHE

ASP

GLU

HIS

THR

LYS

GLY

LEU

ASP

GLY

9

ARG

ASN

VAL

LYS

THR

LEU

VAL

THR

TRP

GLU

10

GLY

ASN

THR

LEU

VAL

CYS

VAL

GLN

LYS

GLY

11

GLU

LYS

GLU

ASN

ARG

GLY

TRP

LYS

GLN

TRP

12

VAL

GLU

GLY

ASP

LYS

LEU

TYR

LEU

GLU

LEU

13

THR

CYS

GLY

ASP

GLN

VAL

CYS

ARG

GLN

VAL

14

PHE

LYS

Entity 2, RTL - C20 H30 O - 286.452 Da.

1

RTL

Samples:

sample_1: Holo cellular retinol-binding protein II, [U-99% 13C; U-99% 15N], 1.0 – 2.0 mM

sample_2: Holo cellular retinol-binding protein II 1.5 mM

sample_3: Holo cellular retinol-binding protein II, [U-99% 15N], 1.5 mM

Ex-con_1: pH: 7.4; temperature: 298 K; ionic strength: 0.081 M

Ex-con_2: pH: 6.5; temperature: 298 K; ionic strength: 0.079 M

Experiments:

Name	Sample	Sample state	Sample conditions

Software:

No software information available

NMR spectrometers:

Varian Unity 500 MHz
Varian UnityPlus 500 MHz

BMRB	4681
PDB	1B4M 1EII 1OPA 1OPB
DBJ	BAB22708
EMBL	CAA52268
GB	AAA42022 AAI25552 AAI25554 EDL20985 EDL77463
REF	NP_033060 NP_036772 XP_006243669
SP	P06768 Q08652
AlphaFold	P06768 Q08652