BMRB Entry 4466

Title:
Chemical Shift assignments for Cu(I) Pseudoazurin from Paracoccus pantotrophus
Deposition date:
1999-11-30
Original release date:
2002-08-09
Authors:
Thompson, Gary; Leung, Yun-Chung; Ferguson, Stuart; Radford, Sheena; Redfield, Christina
Citation:

Citation: Thompson, Gary; Leung, Yun-Chung; Ferguson, Stuart; Radford, Sheena; Redfield, Christina. "The Structure and Dynamics in Solution of Cu(I) Pseudoazurin from Paracoccus pantotrophus."  Protein Sci. 9, 846-858 (2000).

Assembly members:

Assembly members:
pseudoazurin, polymer, 123 residues, 13341.29 Da.
CU1, non-polymer, 63.546 Da.

Natural source:

Natural source:   Common Name: Paracoccus pantotrophus   Taxonomy ID: 82367   Superkingdom: Prokaryote   Kingdom: Monera   Genus/species: Paracoccus pantotrophus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Experimental source:

Natural source:   Common Name: Paracoccus pantotrophus   Taxonomy ID: 82367   Superkingdom: Prokaryote   Kingdom: Monera   Genus/species: Paracoccus pantotrophus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Data sets:
Data typeCount
1H chemical shifts838
15N chemical shifts121

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Pseudoazurin1
2Copper I ion2

Entities:

Entity 1, Pseudoazurin 123 residues - 13341.29 Da.

1   ALATHRHISGLUVALHISMETLEUASNLYS
2   GLYGLUSERGLYALAMETVALPHEGLUPRO
3   ALAPHEVALARGALAGLUPROGLYASPVAL
4   ILEASNPHEVALPROTHRASPLYSSERHIS
5   ASNVALGLUALAILELYSGLUILELEUPRO
6   GLUGLYVALGLUSERPHELYSSERLYSILE
7   ASNGLUSERTYRTHRLEUTHRVALTHRGLU
8   PROGLYLEUTYRGLYVALLYSCYSTHRPRO
9   HISPHEGLYMETGLYMETVALGLYLEUVAL
10   GLNVALGLYASPALAPROGLUASNLEUASP
11   ALAALALYSTHRALALYSMETPROLYSLYS
12   ALAARGGLUARGMETASPALAGLULEUALA
13   GLNVALASN

Entity 2, Copper I ion - Cu - 63.546 Da.

1   CU1

Samples:

pazcu1_h2o_sample1: pseudoazurin, [U-15N], 3 mM; COPPER (I) ION 3 mM; H2O 95%; D2O 5%; Na2S2O4 6.6 mM; PO42- 20 mM

pazcu1_d2o_sample1: pseudoazurin, [U-15N], 3 mM; COPPER (I) ION 3 mM; D2O 100%; Na2S2O4 6.6 mM; PO42- 20 mM

standard_conditions: pH: 6.6; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H - 1H double quantum filtered COSYpazcu1_d2o_sample1not availablestandard_conditions
1H - 1H 60ms TOCSYpazcu1_d2o_sample1not availablestandard_conditions
1H - 1H 200ms NOESYpazcu1_d2o_sample1not availablestandard_conditions

Software:

FELIX v2.3, MSI - raw spectral data (FID) transformations, strip extraction, initial chemical shift measurements

NMRView v2.00 - 4.03, Bruce Johnson, Merck and Co. - peak picking, computer aided assignment, restraint generation

NMR spectrometers:

  • homebuilt . 750 MHz

Related Database Links:

PDB
EMBL CAA93848 CAA97485
GB KGJ09874
REF WP_024842647 WP_036756592
SP P80401
AlphaFold P80401

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks