BMRB Entry 4104

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4104

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C and 15N Chemical Shift Assignments of the 14mer DNA Duplex BS2 in the Complex with the Antennapedia Homeodomain

Deposition date:

1997-01-28

Original release date:

1998-07-14

Authors:

Fernandez, Cesar; Szyperski, Thomas; Ono, Akira; Iwai, Hideo; Tate, Shin-ichi; Kainosho, Masatsune; Wuthrich, Kurt

Citation:

Citation: Fernandez, Cesar; Szyperski, Thomas; Ono, Akira; Iwai, Hideo; Tate, Shin-ichi; Kainosho, Masatsune; Wuthrich, Kurt. "NMR with 13C,15N-doubly-labeled DNA: the Antennapedia Homeodomain Complex with a 14mer DNA Duplex" J. Biomol. NMR ., .-. (1998).

Assembly members:

Assembly members:
BS2_strand_A, polymer, 14 residues, Formula weight is not available
BS2_strand_B, polymer, 14 residues, Formula weight is not available
Antennapedia Homeodomain, polymer, 68 residues, Formula weight is not available

Natural source:

Natural source: Common Name: fruit fly Taxonomy ID: not available Superkingdom: Eukaryota Kingdom: Animalia Genus/species: Drosophila melanogaster

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
BS2_strand_A: GAAAGCCATTAGAG
BS2_strand_B: CTCTAATGGCTTTC
Antennapedia Homeodomain: MRKRGRQTYTRYQTLELEKE FHFNRYLTRRRRIEIAHALS LTERQIKIWFQNRRMKWKKE NKTKGEPG

Data sets:

assigned_chemical_shifts
- chemical_shift_assignment_one

Data type	Count
13C chemical shifts	176
15N chemical shifts	23
1H chemical shifts	257

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	BS2_strand_A	1
2	BS2_strand_B	2
3	AntpHD	3

Entities:

Entity 1, BS2_strand_A 14 residues - Formula weight is not available

1

DG

DA

DG

DC

DA

DT

2

DA

DG

DA

DG

Entity 2, BS2_strand_B 14 residues - Formula weight is not available

1

DC

DT

DC

DT

DA

DT

DG

DC

2

DT

DC

Entity 3, AntpHD 68 residues - Formula weight is not available

1

MET

ARG

LYS

ARG

GLY

ARG

GLN

THR

TYR

THR

2

ARG

TYR

GLN

THR

LEU

GLU

LEU

GLU

LYS

GLU

3

PHE

HIS

PHE

ASN

ARG

TYR

LEU

THR

ARG

4

ARG

ILE

GLU

ILE

ALA

HIS

ALA

LEU

SER

5

LEU

THR

GLU

ARG

GLN

ILE

LYS

ILE

TRP

PHE

6

GLN

ASN

ARG

MET

LYS

TRP

LYS

GLU

7

ASN

LYS

THR

LYS

GLY

GLU

PRO

GLY

Samples:

sample: BS2_strand_A, [U-13C;U-15N], 1.0 mM; BS2_strand_B, [U-13C;U-15N], 1.0 mM; Antennapedia Homeodomain, [U-15N], 1.0 mM; D2O 10%; H2O 90%

sample_conditions: pH: 6.0; temperature: 309 K

Experiments:

Name	Sample	Sample state	Sample conditions
not available	sample	not available	sample_conditions

Software:

No software information available

NMR spectrometers:

Bruker DRX500 500 MHz
Bruker DRX600 600 MHz
Varian Unity Plus Unity Plus 750 MHz

BMRB	1037
PDB	1AHD 1HOM 1SAN 2HOA 9ANT
DBJ	BAA04087 BAE97003 BAM20301
EMBL	CAA27417 CAA43307 CAB75740 CCA94746
GB	AAA28373 AAA28376 AAA28737 AAA68461 AAA70214
PIR	H45186
PRF	1205216A
REF	NP_001034505 NP_001037319 NP_001161164 NP_996166 NP_996167
SP	P02833 Q24645
AlphaFold	P02833 Q24645