BMRB Entry 4079

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4079

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Structure of the A-Domain of HMG1 and Its Interaction with DNA As Studied by Heteronuclear Three- and Four-Dimensional NMR Spectroscopy

Deposition date:

1997-12-07

Original release date:

2001-02-17

Authors:

Hardman, Colin; Broadhurst, R.; Raine, Andrew; Grasser, Klaus; Thomas, Jean; Laue, Ernest

Citation:

Citation: Hardman, Colin; Broadhurst, R.; Raine, Andrew; Grasser, Klaus; Thomas, Jean; Laue, Ernest. "Structure of the A-Domain of HMG1 and Its Interaction with DNA As Studied by Heteronuclear Three- and Four-Dimensional NMR Spectroscopy" Biochemistry 34, 16596-16607 (1995).

Assembly members:

Assembly members:
A-domain of HMG1, polymer, 83 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Norway rat Taxonomy ID: 10116 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rattus norvegicus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pT7-7-HMG1-A or pT7-7-HMG1-A(C22S)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
A-domain of HMG1: GKGDPKKPRGKMSSYAFFVQ TSREEHKKKHPDASVNFSEF SKKCSERWKTMSAKEKGKFE DMAKADKARYEREMKTYIPP KGE

Data sets:

assigned_chemical_shifts
- chemical_shift_assignment

Data type	Count
13C chemical shifts	305
15N chemical shifts	79
1H chemical shifts	581

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	A-domain HMG1	1

Entities:

Entity 1, A-domain HMG1 83 residues - Formula weight is not available

1

GLY

LYS

GLY

ASP

PRO

LYS

PRO

ARG

GLY

2

LYS

MET

SER

TYR

ALA

PHE

VAL

GLN

3

THR

SER

ARG

GLU

HIS

LYS

HIS

4

PRO

ASP

ALA

SER

VAL

ASN

PHE

SER

GLU

PHE

5

SER

LYS

CYS

SER

GLU

ARG

TRP

LYS

THR

6

MET

SER

ALA

LYS

GLU

LYS

GLY

LYS

PHE

GLU

7

ASP

MET

ALA

LYS

ALA

ASP

LYS

ALA

ARG

TYR

8

GLU

ARG

GLU

MET

LYS

THR

TYR

ILE

PRO

9

LYS

GLY

GLU

Samples:

sample_one: A-domain of HMG12 – 5 mM; D2O 10%; H2O 90%; sodium phosphate 10 mM; NaCl 0.15 M; DTT 50 uM; NaN3 .02%

sample_two: A-domain of HMG1, [U-15N], 2 – 5 mM; D2O 10%; H2O 90%; sodium phosphate 10 mM; NaCl 0.15 M; DTT 50 uM; NaN3 .02%

sample_three: A-domain of HMG1, [U-13C], 2 – 5 mM; D2O 10%; H2O 90%; sodium phosphate 10 mM; NaCl 0.15 M; DTT 50 uM; NaN3 .02%

sample_four: A-domain of HMG1, [U-15N;U-13C], 2 – 5 mM; D2O 10%; H2O 90%; sodium phosphate 10 mM; NaCl 0.15 M; DTT 50 uM; NaN3 .02%

sample_conditions: pH: 5.0; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
not available	not available	not available	sample_conditions

Software:

No software information available

NMR spectrometers:

Bruker AMX 600 MHz

BMRB	11147 11532 15148 15149 15502 18709
PDB	1AAB 1CKT 2LY4 2RTU 2YRQ 4QR9
DBJ	BAA09924 BAC29902 BAC34367 BAC34773 BAC38678
EMBL	CAA31110 CAA31284 CAA56631 CAA68441 CAA68526
GB	AAA20508 AAA31050 AAA40729 AAA57042 AAA64970
PIR	S29857
REF	NP_001002937 NP_001004034 NP_001075304 NP_001102843 NP_001162380
SP	A9RA84 B0CM99 B1MTB0 P07156 P09429
TPG	DAA23902
AlphaFold	A9RA84 B0CM99 B1MTB0 P07156 P09429