BMRB Entry 15502
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR15502
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Watson, Matthew; Stott, Katherine; Thomas, Jean. "Mapping intramolecular interactions between domains in HMGB1 using a tail-truncation approach" J. Mol. Biol. 374, 1286-1297 (2007).
PubMed: 17988686
Assembly members:
HMGB1, polymer, 214 residues, Formula weight is not available
Natural source: Common Name: Rat Taxonomy ID: 10116 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rattus norvegicus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pBAT4
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 173 |
| 1H chemical shifts | 179 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | HMGB1 | 1 |
Entities:
Entity 1, HMGB1 214 residues - Formula weight is not available
| 1 | GLY | LYS | GLY | ASP | PRO | LYS | LYS | PRO | ARG | GLY | ||||
| 2 | LYS | MET | SER | SER | TYR | ALA | PHE | PHE | VAL | GLN | ||||
| 3 | THR | CYS | ARG | GLU | GLU | HIS | LYS | LYS | LYS | HIS | ||||
| 4 | PRO | ASP | ALA | SER | VAL | ASN | PHE | SER | GLU | PHE | ||||
| 5 | SER | LYS | LYS | CYS | SER | GLU | ARG | TRP | LYS | THR | ||||
| 6 | MET | SER | ALA | LYS | GLU | LYS | GLY | LYS | PHE | GLU | ||||
| 7 | ASP | MET | ALA | LYS | ALA | ASP | LYS | ALA | ARG | TYR | ||||
| 8 | GLU | ARG | GLU | MET | LYS | THR | TYR | ILE | PRO | PRO | ||||
| 9 | LYS | GLY | GLU | THR | LYS | LYS | LYS | PHE | LYS | ASP | ||||
| 10 | PRO | ASN | ALA | PRO | LYS | ARG | PRO | PRO | SER | ALA | ||||
| 11 | PHE | PHE | LEU | PHE | CYS | SER | GLU | TYR | ARG | PRO | ||||
| 12 | LYS | ILE | LYS | GLY | GLU | HIS | PRO | GLY | LEU | SER | ||||
| 13 | ILE | GLY | ASP | VAL | ALA | LYS | LYS | LEU | GLY | GLU | ||||
| 14 | MET | TRP | ASN | ASN | THR | ALA | ALA | ASP | ASP | LYS | ||||
| 15 | GLN | PRO | TYR | GLU | LYS | LYS | ALA | ALA | LYS | LEU | ||||
| 16 | LYS | GLU | LYS | TYR | GLU | LYS | ASP | ILE | ALA | ALA | ||||
| 17 | TYR | ARG | ALA | LYS | GLY | LYS | PRO | ASP | ALA | ALA | ||||
| 18 | LYS | LYS | GLY | VAL | VAL | LYS | ALA | GLU | LYS | SER | ||||
| 19 | LYS | LYS | LYS | LYS | GLU | GLU | GLU | ASP | ASP | GLU | ||||
| 20 | GLU | ASP | GLU | GLU | ASP | GLU | GLU | GLU | GLU | GLU | ||||
| 21 | GLU | GLU | GLU | ASP | GLU | ASP | GLU | GLU | GLU | ASP | ||||
| 22 | ASP | ASP | ASP | GLU |
Samples:
sample_1: HMGB1, [U-98% 15N], 0.3-1.4 mM
sample_conditions_1: ionic strength: 0.001 M; pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N TOCSY | sample_1 | isotropic | sample_conditions_1 |
Software:
Analysis, CCPN - chemical shift assignment, data analysis, peak picking
NMR spectrometers:
- Bruker DRX 500 MHz
- Bruker DRX 800 MHz
Related Database Links:
| BMRB | 11147 15148 15149 |
| PDB | |
| DBJ | BAA09924 BAC29902 BAC34367 BAC34773 BAC38678 |
| EMBL | CAA31110 CAA31284 CAA56631 CAA68441 CAA68526 |
| GB | AAA20508 AAA31050 AAA40729 AAA57042 AAA64970 |
| PIR | S29857 |
| REF | NP_001002937 NP_001004034 NP_001075304 NP_001102843 NP_001162380 |
| SP | A9RA84 B0CM99 B1MTB0 P07156 P09429 |
| TPG | DAA21468 DAA23902 |
| AlphaFold | A9RA84 B0CM99 B1MTB0 P07156 P09429 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks