BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 15148

Title: HMGB1 AB boxes + basic tail backbone assignment   PubMed: 17462578

Deposition date: 2007-02-26 Original release date: 2007-06-05

Authors: Mollica, Luca; Musco, Francesco; Spitaleri, Andrea; Dallacosta, Corrado; Zamai, Moreno; Agresti, Alessandra; Trisciuoglio, Lisa; Bianchi, Marco; Musco, Giovanna

Citation: Mollica, Luca; De Marchis, Francesco; Spitaleri, Andrea; Dallacosta, Corrado; Pennacchini, D.; Zamai, Moreno; Agresti, Alessandra; Trisciuoglio, Lisa; Musco, Giovanna; Bianchi, Marco. "Glycyrrhizin binds to high-mobility group box 1 protein and inhibits its cytokine activities"  Chem. Biol. 14, 431-441 (2007).

Assembly members:
HMGB1_ABbtail, polymer, 187 residues, Formula weight is not available

Natural source:   Common Name: not available   Taxonomy ID: 562   Superkingdom: not available   Kingdom: Escherichia   Genus/species: coli not available

Experimental source:   Production method: recombinant technology' 'Escherichia coli

Entity Sequences (FASTA):
HMGB1_ABbtail: GKGDPKKPRGKMSSYAFFVQ TCREEHKKKHPDASVNFSEF SKKCSERWKTMSAKEKGKFE DMAKADKARYEREMKTYIPP KGETKKKFKDPNAPKRPPSA FFLFCSEYRPKIKGEHPGLS IGDVAKKLGEMWNNTAADDK QPYEKKAAKLKEKYEKDIAA YRAKGKPDAAKKGVVKAEKS KKKKEEE

Data sets:
Data typeCount
13C chemical shifts336
15N chemical shifts174
1H chemical shifts174

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1HMGB1 ABbtail1

Entities:

Entity 1, HMGB1 ABbtail 187 residues - Formula weight is not available

1   GLYLYSGLYASPPROLYSLYSPROARGGLY
2   LYSMETSERSERTYRALAPHEPHEVALGLN
3   THRCYSARGGLUGLUHISLYSLYSLYSHIS
4   PROASPALASERVALASNPHESERGLUPHE
5   SERLYSLYSCYSSERGLUARGTRPLYSTHR
6   METSERALALYSGLULYSGLYLYSPHEGLU
7   ASPMETALALYSALAASPLYSALAARGTYR
8   GLUARGGLUMETLYSTHRTYRILEPROPRO
9   LYSGLYGLUTHRLYSLYSLYSPHELYSASP
10   PROASNALAPROLYSARGPROPROSERALA
11   PHEPHELEUPHECYSSERGLUTYRARGPRO
12   LYSILELYSGLYGLUHISPROGLYLEUSER
13   ILEGLYASPVALALALYSLYSLEUGLYGLU
14   METTRPASNASNTHRALAALAASPASPLYS
15   GLNPROTYRGLULYSLYSALAALALYSLEU
16   LYSGLULYSTYRGLULYSASPILEALAALA
17   TYRARGALALYSGLYLYSPROASPALAALA
18   LYSLYSGLYVALVALLYSALAGLULYSSER
19   LYSLYSLYSLYSGLUGLUGLU

Samples:

sample_1: HMGB1 ABbtail' '[U-100% 13C; U-100% 15N] 0.1; .

sample_conditions_1: ionic strength: 150 mM; pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Bruker AMX 500 MHz
  • Bruker AMX 600 MHz

Related Database Links:

BMRB 11147 15149 15502
PDB
DBJ BAA09924 BAC29902 BAC34367 BAC34773 BAC38678
EMBL CAA31110 CAA31284 CAA56631 CAA68441 CAA68526
GB AAA20508 AAA31050 AAA40729 AAA57042 AAA64970
PIR S29857
REF NP_001002937 NP_001004034 NP_001075304 NP_001102843 NP_001162380
SP A9RA84 B0CM99 B1MTB0 P07156 P09429
TPG DAA21468 DAA23902

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts