BMRB Entry 4032
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4032
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Citation: Shimotakahara, Sakurako; Rios, Carlos; Laity, John; Zimmerman, Diane; Scheraga, Harold; Montelione, Gaetano. "NMR Structural Analysis of an Analog of an Intermediate Formed in the
Rate-Determining Step of One Pathway in the Oxidative Folding of Bovine
Pancreatic Ribonuclease A: Automated Analysis of 1H, 13C and 15N Resonance
Assignments for Wild-Type and [C65S, C72S] Mutant Forms." Biochemistry 36, 6915-6929 (1997).
PubMed: 9188686
Assembly members:
bovine pancreatic [C65S,C72S] ribonuclease A, polymer, 124 residues, Formula weight is not available
Natural source: Common Name: 9913 Taxonomy ID: Eukaryota Superkingdom: Metazoa Kingdom: not available Genus/species: Bos taurus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: plasmid
Entity Sequences (FASTA):
bovine pancreatic [C65S,C72S] ribonuclease A: KETAAAKFERQHMDSSTSAA
SSSNYCNQMMKSRNLTKDRC
KPVNTFVHESLADVQAVCSQ
KNVASKNGQTNSYQSYSTMS
ITDCRETGSSKYPNCAYKTT
QANKHIIVACEGNPYVPVHF
DASV
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 363 |
| 15N chemical shifts | 119 |
| 1H chemical shifts | 244 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | bovine pancreatic [C65S,C72S] ribonuclease A | 1 |
Entities:
Entity 1, bovine pancreatic [C65S,C72S] ribonuclease A 124 residues - Formula weight is not available
| 1 | LYS | GLU | THR | ALA | ALA | ALA | LYS | PHE | GLU | ARG | ||||
| 2 | GLN | HIS | MET | ASP | SER | SER | THR | SER | ALA | ALA | ||||
| 3 | SER | SER | SER | ASN | TYR | CYS | ASN | GLN | MET | MET | ||||
| 4 | LYS | SER | ARG | ASN | LEU | THR | LYS | ASP | ARG | CYS | ||||
| 5 | LYS | PRO | VAL | ASN | THR | PHE | VAL | HIS | GLU | SER | ||||
| 6 | LEU | ALA | ASP | VAL | GLN | ALA | VAL | CYS | SER | GLN | ||||
| 7 | LYS | ASN | VAL | ALA | SER | LYS | ASN | GLY | GLN | THR | ||||
| 8 | ASN | SER | TYR | GLN | SER | TYR | SER | THR | MET | SER | ||||
| 9 | ILE | THR | ASP | CYS | ARG | GLU | THR | GLY | SER | SER | ||||
| 10 | LYS | TYR | PRO | ASN | CYS | ALA | TYR | LYS | THR | THR | ||||
| 11 | GLN | ALA | ASN | LYS | HIS | ILE | ILE | VAL | ALA | CYS | ||||
| 12 | GLU | GLY | ASN | PRO | TYR | VAL | PRO | VAL | HIS | PHE | ||||
| 13 | ASP | ALA | SER | VAL |
Samples:
sample_one: bovine pancreatic [C65S,C72S] ribonuclease A, [U-15N], 2.7 mM; D2O 10%; H2O 90%; NaCl 0.0 mM; KCl 0.0 mM
sample_two: bovine pancreatic [C65S,C72S] ribonuclease A, [U-15N;U-13C], 3.0 mM; D2O 10%; H2O 90%
sample_conditions_one: pH*: 4.6; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|
Software:
No software information available
NMR spectrometers:
- unknown unknown 0 MHz
Related Database Links:
| BMRB | 1072 16010 16011 16503 16742 17099 17172 19065 2928 385 4031 443 |
| PDB | |
| EMBL | CAA30263 CAA33801 CAB37066 |
| GB | AAA72269 AAA72757 AAB35594 AAB36134 AAI49530 |
| PIR | JC5560 NRBOB |
| PRF | 630436A |
| REF | NP_001014408 XP_005211519 XP_005901936 XP_010837737 |
| SP | P61823 P61824 |
| TPE | CDG32088 |
| TPG | DAA25470 |
| AlphaFold | P61823 P61824 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
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SPARKY: Backbone
or all simulated peaks