BMRB Entry 17099
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17099
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Citation: Brauer, Manfred; Benz, F.. "1H NMR Studies of the Binding of EDTA to Bovine Pancreatic Ribonuclease" Biochim. Biophys. Acta 533, 186-194 (1978).
PubMed: 416852
Assembly members:
ribonuclease, polymer, 124 residues, 292.243 Da.
{[-(BIS-CARBOXYMETHYL-AMINO)-ETHYL]-CARBOXYMETHYL-AMINO}-ACETIC ACID, non-polymer, 292.243 Da.
Natural source: Common Name: Cattle Taxonomy ID: 9913 Superkingdom: Eukaryota Kingdom: Metaoza Genus/species: Bos taurus
Experimental source: Production method: purified from the natural source
Entity Sequences (FASTA):
ribonuclease: KETAAAKFERQHMDSSTSAA
SSSNYCNQMMKSRNLTKDRC
KPVNTFVHESLADVQAVCSQ
KNVACKNGQTNCYQSYSTMS
ITDCRETGSSKYPNCAYKTT
QANKHIIVACEGNPYVPVHF
DASV
- binding_constants
- pKa_values
| Data type | Count |
| binding constants | 1 |
| pKa values | 4 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | ribonuclease | 1 |
| 2 | EDTA | 2 |
Entities:
Entity 1, ribonuclease 124 residues - 292.243 Da.
| 1 | LYS | GLU | THR | ALA | ALA | ALA | LYS | PHE | GLU | ARG | ||||
| 2 | GLN | HIS | MET | ASP | SER | SER | THR | SER | ALA | ALA | ||||
| 3 | SER | SER | SER | ASN | TYR | CYS | ASN | GLN | MET | MET | ||||
| 4 | LYS | SER | ARG | ASN | LEU | THR | LYS | ASP | ARG | CYS | ||||
| 5 | LYS | PRO | VAL | ASN | THR | PHE | VAL | HIS | GLU | SER | ||||
| 6 | LEU | ALA | ASP | VAL | GLN | ALA | VAL | CYS | SER | GLN | ||||
| 7 | LYS | ASN | VAL | ALA | CYS | LYS | ASN | GLY | GLN | THR | ||||
| 8 | ASN | CYS | TYR | GLN | SER | TYR | SER | THR | MET | SER | ||||
| 9 | ILE | THR | ASP | CYS | ARG | GLU | THR | GLY | SER | SER | ||||
| 10 | LYS | TYR | PRO | ASN | CYS | ALA | TYR | LYS | THR | THR | ||||
| 11 | GLN | ALA | ASN | LYS | HIS | ILE | ILE | VAL | ALA | CYS | ||||
| 12 | GLU | GLY | ASN | PRO | TYR | VAL | PRO | VAL | HIS | PHE | ||||
| 13 | ASP | ALA | SER | VAL |
Entity 2, EDTA - C10 H16 N2 O8 - 292.243 Da.
| 1 | EDT |
Samples:
sample_1: deuteroacetate buffer 0.2 M; ribonuclease 2.0 mM; EDTA0 – 63.4 mM; D2O 100%
sample_2: deuterocetate buffer 0.2 M; ribonuclease 2.0 mM; EDTA 63.4 mM; D2O 100%
sample_conditions_1: pH: 5.5; pressure: 1 atm; temperature: 307 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H NMR | sample_1 | isotropic | sample_conditions_1 |
| 1H NMR | sample_2 | isotropic | sample_conditions_1 |
Software:
none_given, na - na
NMR spectrometers:
- Bruker WH270 270 MHz
Related Database Links:
| BMRB | 1072 16010 16011 16503 16742 17172 19065 2928 385 4031 4032 443 |
| PDB | |
| EMBL | CAA30263 CAA33801 CAB37066 |
| GB | AAA72269 AAA72757 AAB35594 AAB36134 AAI49530 |
| PIR | JC5560 NRBOB |
| PRF | 630436A |
| REF | NP_001014408 XP_005211519 XP_005901936 XP_010837737 |
| SP | P61823 P61824 |
| TPE | CDG32088 |
| TPG | DAA25470 |
| AlphaFold | P61823 P61824 |