BMRB Entry 17172
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17172
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
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Citation: Lopez, Jorge Pedro; Bruix, Marta; Font, Josep; Rib, Marc; Vilanova, Maria; Rico, Manuel; Gotte, Giovanni; Libonati, Massimo; Gonz lez, Carlos; Laurentr, Douglas. "Formation, structure and dissociation of the RNase S 3D domain-swapped dimer." J. Biol. Chem. 281, 9400-9406 (2006).
PubMed: 16415350
Assembly members:
RNase_A_C-dimer, polymer, 124 residues, 13684 Da.
Natural source: Common Name: Cattle Taxonomy ID: 9913 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Bos taurus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: unknown
Entity Sequences (FASTA):
RNase_A_C-dimer: KETAAAKFERQHMDSSTSAA
SSSNYCNQMMKSRNLTKDRC
KPVNTFVHESLADVQAVCSQ
KNVACKNGQTNCYQSYSTMS
ITDCRETGSSKYPNCAYKTT
QANKHIIVACEGNPYVPVHF
DASV
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 98 |
| 1H chemical shifts | 101 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | RNase A C-dimer, chain 1 | 1 |
| 2 | RNase A C-dimer, chain 2 | 1 |
Entities:
Entity 1, RNase A C-dimer, chain 1 124 residues - 13684 Da.
| 1 | LYS | GLU | THR | ALA | ALA | ALA | LYS | PHE | GLU | ARG | ||||
| 2 | GLN | HIS | MET | ASP | SER | SER | THR | SER | ALA | ALA | ||||
| 3 | SER | SER | SER | ASN | TYR | CYS | ASN | GLN | MET | MET | ||||
| 4 | LYS | SER | ARG | ASN | LEU | THR | LYS | ASP | ARG | CYS | ||||
| 5 | LYS | PRO | VAL | ASN | THR | PHE | VAL | HIS | GLU | SER | ||||
| 6 | LEU | ALA | ASP | VAL | GLN | ALA | VAL | CYS | SER | GLN | ||||
| 7 | LYS | ASN | VAL | ALA | CYS | LYS | ASN | GLY | GLN | THR | ||||
| 8 | ASN | CYS | TYR | GLN | SER | TYR | SER | THR | MET | SER | ||||
| 9 | ILE | THR | ASP | CYS | ARG | GLU | THR | GLY | SER | SER | ||||
| 10 | LYS | TYR | PRO | ASN | CYS | ALA | TYR | LYS | THR | THR | ||||
| 11 | GLN | ALA | ASN | LYS | HIS | ILE | ILE | VAL | ALA | CYS | ||||
| 12 | GLU | GLY | ASN | PRO | TYR | VAL | PRO | VAL | HIS | PHE | ||||
| 13 | ASP | ALA | SER | VAL |
Samples:
sample_1: RNase A C-dimer, [U-99% 15N], 0.08 – 1.0 mM; Na2HPO4 0.10 M; NaH2PO4 0.10 M; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 0.5 M; pH: 6.7; pressure: 1 atm; temperature: 298.0 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN, Bruker Biospin, Goddard - collection
SPARKY, Goddard - chemical shift assignment
NMR spectrometers:
- Bruker Avance 800 MHz
Related Database Links:
| BMRB | 1072 16010 16011 16503 16742 17099 19065 2928 385 4031 4032 443 |
| PDB | |
| EMBL | CAA30263 CAA33801 CAB37066 |
| GB | AAA72269 AAA72757 AAB35594 AAB36134 AAI49530 |
| PIR | JC5560 NRBOB |
| PRF | 630436A |
| REF | NP_001014408 XP_005211519 XP_005901936 XP_010837737 |
| SP | P61823 P61824 |
| TPE | CDG32088 |
| TPG | DAA25470 |
| AlphaFold | P61823 P61824 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks