BMRB Entry 3322

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR3322
MolProbity Validation Chart

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Title:
Characterisation of a cellulosome dockerin domain from the anaerobic fungus Piromyces equi
Deposition date:
2001-09-10
Original release date:
1995-07-31
Authors:
Raghothama, Srinivasrao; Eberhardt, Ruth; Simpson, Peter; Wigelsworth, Darran; White, Peter; Hazlewood, Geoffrey; Nagy, Tibor; Gilbert, Harry; Williamson, Michael
Citation:

Citation: Raghothama, Srinivasrao; Eberhardt, Ruth; Simpson, Peter; Wigelsworth, Darran; White, Peter; Hazlewood, Geoffrey; Nagy, Tibor; Gilbert, Harry; Williamson, Michael. "Characterisation of a cellulosome dockerin domain from the anaerobic fungus Piromyces equi"  Nat. Struct. Biol. 8, 775-778 (2001).
PubMed: 11524680

Assembly members:

Assembly members:
dockerin, polymer, 52 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Piromyces equi   Taxonomy ID: 99929   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Piromyces equi

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX-4T-2

Entity Sequences (FASTA):

Entity Sequences (FASTA):
dockerin: GSCWAQSQGYNCCNNPSSTK VEYTDASGQWGVQNGQWCGI DYSYGQNQGNES

Data sets:
  • assigned_chemical_shifts
Data typeCount
15N chemical shifts62
1H chemical shifts301

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1dockerin1

Entities:

Entity 1, dockerin 52 residues - Formula weight is not available

1   GLYSERCYSTRPALAGLNSERGLNGLYTYR
2   ASNCYSCYSASNASNPROSERSERTHRLYS
3   VALGLUTYRTHRASPALASERGLYGLNTRP
4   GLYVALGLNASNGLYGLNTRPCYSGLYILE
5   ASPTYRSERTYRGLYGLNASNGLNGLYASN
6   GLUSER

Samples:

Sample_1: dockerin, [U-15N], 1 mM

condition_1: pH: 6.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D-HSQC/NOESYSample_1not availablecondition_1
HNHASample_1not availablecondition_1
HNHBSample_1not availablecondition_1
2D-HSQCSample_1not availablecondition_1
15N decoupled NOESYSample_1not availablecondition_1
TOCSYSample_1not availablecondition_1
DQF-COSYSample_1not availablecondition_1
E.COSYSample_1not availablecondition_1

Software:

FELIX -

NMR spectrometers:

  • Bruker DRX 500 MHz
  • Bruker DRX 600 MHz

Related Database Links:

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