BMRB Entry 31061

Name: Structure of pre-miR-31 reveals an active role in Dicer processing
Published: 2023-02-14

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PDB ID: 8fcs
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR31061
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Structure of pre-miR-31 reveals an active role in Dicer processing

Deposition date:

2022-12-01

Original release date:

2023-02-14

Authors:

Kotar, A.; Ma, S.; Keane, S.

Citation:

Citation: Ma, Sicong; Kotar, Anita; Hall, Ian; Grote, Scott; Rouskin, Silvi; Keane, Sarah. "Structure of pre-miR-31 reveals an active role in Dicer-TRBP complex processing" Proc. Natl. Acad. Sci. U. S. A. 120, e2300527120-e2300527120 (2023).
PubMed: 37725636

Assembly members:

Assembly members:
entity_1, polymer, 71 residues, 22872.592 Da.

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GGAGAGGAGGCAAGAUGCUG GCAUAGCUGUUGAACUGGGA ACCUGCUAUGCCAACAUAUU GCCAUCUUUCC

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	54
1H chemical shifts	329

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	unit_1	1

Entities:

Entity 1, unit_1 71 residues - 22872.592 Da.

1

G

A

G

A

G

A

G

2

C

A

G

A

U

G

C

U

G

3

G

C

A

U

A

G

C

U

G

U

4

U

G

A

C

U

G

A

5

A

C

U

G

C

U

A

U

G

6

C

A

C

A

U

A

U

7

G

C

A

U

C

U

C

8

C

Name	Sample	Sample state	Sample conditions
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_2	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_3	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_4	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_5	isotropic	sample_conditions_1
2D 1H-13C IPAP-HSQC	sample_9	isotropic	sample_conditions_2
2D 1H-15N BESTsellr HNN COSY	sample_6	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_7	isotropic	sample_conditions_2
2D 1H-13C HSQC	sample_6	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-13C IPAP-HSQC	sample_10	anisotropic	sample_conditions_2
2D 1H-13C HSQC	sample_8	isotropic	sample_conditions_1

BMRB Entry 31061

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers: