BMRB Entry 25735

Name: The selective autophagy receptor TAX1BP1 is required for autophagy-dependent capture of cytosolic Salmonella typhimurium
Published: 2015-09-21

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PDB ID: 5aas
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25735
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

The selective autophagy receptor TAX1BP1 is required for autophagy-dependent capture of cytosolic Salmonella typhimurium

Deposition date:

2015-07-29

Original release date:

2015-09-21

Authors:

Tumbarello, D; Manna, P; Allen, Mark; Bycroft, M; Kendrick-jones, J; Buss, F

Citation:

Citation: Tumbarello, David; Manna, Paul; Allen, Mark; Bycroft, Mark; Arden, Susan; Kendrick-Jones, John; Buss, Folma. "The Autophagy Receptor TAX1BP1 and the Molecular Motor Myosin VI Are Required for Clearance of Salmonella Typhimurium by Autophagy" PLoS Pathog. 11, e1005174-e1005174 (2015).
PubMed: 26451915

Assembly members:

Assembly members:
TAXBP1, polymer, 64 residues, 7679.4363 Da.
ZINC ION, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: ESCHERICHIA COLI BL21(DE3) Vector: HLTV

Entity Sequences (FASTA):

Entity Sequences (FASTA):
TAXBP1: GSSFDVHKKCPLCELMFPPN YDQSKFEEHVESHWKVCPMC SEQFPPDYDQQVFERHVQTH FDQN

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list

Data type	Count
13C chemical shifts	179
15N chemical shifts	63
1H chemical shifts	436

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	TAXBP1	1
2	ZINC ION, 1	2
3	ZINC ION, 2	2

Entities:

Entity 1, TAXBP1 64 residues - 7679.4363 Da.

1

GLY

SER

PHE

ASP

VAL

HIS

LYS

CYS

2

PRO

LEU

CYS

GLU

LEU

MET

PHE

PRO

ASN

3

TYR

ASP

GLN

SER

LYS

PHE

GLU

HIS

VAL

4

GLU

SER

HIS

TRP

LYS

VAL

CYS

PRO

MET

CYS

5

SER

GLU

GLN

PHE

PRO

ASP

TYR

ASP

GLN

6

GLN

VAL

PHE

GLU

ARG

HIS

VAL

GLN

THR

HIS

7

PHE

ASP

GLN

ASN

Entity 2, ZINC ION, 1 - 65.409 Da.

1

ZN

Samples:

sample_1: TAXBP1, [U-13C; U-15N], 1.5 mM; H2O 95%; D2O 5%

sample_conditions_1: ionic strength: 100.000 mM; pH: 6.500; pressure: 1.000 atm; temperature: 298.000 K

Experiments:

Name	Sample	Sample state	Sample conditions
NOESY	sample_1	solution	sample_conditions_1
TOCSY	sample_1	solution	sample_conditions_1
DQF-COSY	sample_1	solution	sample_conditions_1
HSQC	sample_1	solution	sample_conditions_1
HNCACB	sample_1	solution	sample_conditions_1
CBCACONH	sample_1	solution	sample_conditions_1
HNCO	sample_1	solution	sample_conditions_1
HNCACO	sample_1	solution	sample_conditions_1
HNHB	sample_1	solution	sample_conditions_1

Software:

Ansig vany, Kraulis - chemical shift assignment

AutoDep v4.3, PDBe - collection

CNS vany, Brunger, Adams, Clore, Gros, Nilges and Read - chemical shift assignment

NMR spectrometers:

Bruker Avance 800 MHz

UNP	TAXB1_HUMAN
AlphaFold	Q9UI88